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A simple filter-paper technique allows detection of mucosal cytokine levelsin vivo in ulcerative colitis

Interleukin-1 and interleukin-1-receptor antagonist

  • Intestinal Disorders, Inflammatory Bowel Disease, Immunology, And Microbiology
  • Published:
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Abstract

A simple filter-paper method forin vivo assessment of cytokines in intestinal mucosa of patients with ulcerative colitis (UC) was developed and evaluated. Twenty-eight patients were included. Twenty-three patients had ulcerative colitis (UC) and five irritable bowel syndrome (IBS). Inflammation was assessed endoscopically. Through a rectoscope, filter paper was applied to the macroscopically most inflamed area of the rectal mucosa until soaked. The filter paper was transferred to a buffer solution, and IL-1β and IL-1ra were assessed using ELISA. Positive correlations between endoscopic grading and ln(IL-1β) (P<0.0001) and ln(IL-1ra) (P<0.001) and a negative correlation between endoscopic grading and ln(IL-1ra/IL-1β) (P<0.02) were found. In measurements during and after a flare-up no significant change in IL-1ra but a significant decrease in IL-1β was detected, which is in agreement with investigations on biopsies. In conclusion, the filter-paper technique is an easily applicable, low-risk method that provides a means of monitoring cytokinesin vivo in UC.

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References

  1. Bendtzen K: Cytokines and natural regulators of cytokines. Immunol Lett 43:111–123, 1994

    PubMed  Google Scholar 

  2. Dinarello CA, Wolff SM: Mechanisms of disease: The role of interleukin-1 in disease. N Engl J Med 328(2):106–113, 1993

    PubMed  Google Scholar 

  3. Macdermott RP: Alterations in the mucosal immune system in ulcerative colitis and Crohn's disease. Med Clin North Am 78(6):1207–1231, 1994

    PubMed  Google Scholar 

  4. Brynskov J, Tvede N, Andersen CB, Vilien M: Increased concentrations of interleukin-1β, interleukin-2, and soluble interleukin-2 receptors in endoscopical mucosal biopsy specimens with active inflammatory bowel disease. Gut 33:55–58, 1992

    PubMed  Google Scholar 

  5. Ligumsky M: Role of interleukin-1 in inflammatory bowel disease—enhanced production during active disease. Gut 31:686–689, 1990

    PubMed  Google Scholar 

  6. Isaacs KL, Sartor RB, Haskill S: Cytokine messenger RNA profiles in inflammatory bowel disease mucosa detected by polymerase chain reaction amplification. Gastroenterology 103:1587–1595, 1992

    PubMed  Google Scholar 

  7. Stevens C, Walz G, Singaram C, Lipman ML, Zankar B, Muggia A: Tumor necrosis factor a, interleukin-1β, and interleukin-6 in inflammatory bowel disease. Dig Dis Sci 37:26, 1992

    Google Scholar 

  8. Mahida YR, Wu K, Jewell DP: Enhanced production of interleukin-1β by mononuclear cells isolated from mucosa with active ulcerative colitis or Crohn's disease. Gut 30:835–838, 1989

    PubMed  Google Scholar 

  9. Alam R, Sim TC, Hilsmeier K, Grant JA: Development of a new technique for recovery of cytokines from inflammatory sitesin situ. J Immunol Methods 155:25–29, 1992

    PubMed  Google Scholar 

  10. Langholz E, Munkholm P, Nielsen OH, Kreiner S, Binder V: Incidence and prevalence of ulcerative colitis in Copenhagen county from 1962 to 1987. Scand J Gastroenterol 26:1247–1256, 1991

    PubMed  Google Scholar 

  11. Søltoft J, Gudmand-Høyer E, Krag E, Christensen E, Wulff HR: A double-blind trial of the effect of wheat bran on symptoms of irritable bowel syndrome. Lancet 1:270–272, 1976

    PubMed  Google Scholar 

  12. Binder V: A comparison between clinical state, macroscopic and microscopic appearances of rectal mucosa, and cytologic picture of mucosal exudate in ulcerative colitis. Scand J Gastroenterol 5:627–632, 1970

    PubMed  Google Scholar 

  13. Brynskov J, Nielsen OH, Ahnfelt-Rønne I, Bendtzen K: Cytokines in inflammatory bowel disease. Scand J Gastroenterol 27:897–906, 1992

    PubMed  Google Scholar 

  14. Brynskov J, Nielsen OH, Ahnfelt-Rønne I, Bendtzen K: Cytokines (immunoinflammatory hormones) and their natural regulation in inflammatory bowel disease (Crohn's disease and ulcerative colitis): A review. Dig Dis 12:290–304, 1994

    PubMed  Google Scholar 

  15. Hannum CH: Interleukin-1 receptor antagonist activity of a human interleukin-1 inhibitor. Nature 343:336–340, 1990

    PubMed  Google Scholar 

  16. Mansfield JC, Holden H, Tarlow JK, Di Giovine FS, McDowell TL, Wilson AG, Holdsworth CD, Duff GW: Novel genetic association between ulcerative colitis and the anti-inflammatory cytokine interleukin-1 receptor antagonist. Gastroenterology 106:637–642, 1994

    PubMed  Google Scholar 

  17. Cominelli F, Nast CC, Duchini A, Lee M: Recombinant interleukin-1 receptor antagonist blocks the proinflammatory activity of endogenous interleukin-1 in rabbit immune colitis. Gastroenterology 103:65–71, 1992

    PubMed  Google Scholar 

  18. Nishiyama T, Mitsuyama K, Toyonaga A, Sasaki E, Tanikawa K: Colonic mucosal interleukin 1 receptor antagonist in inflammatory bowel disease. Digestion 55:368–373, 1994

    PubMed  Google Scholar 

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This work was sponsored by P. Carl Petersens fond and Fonden af 1860.

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Hendel, J., Nielsen, O.H., Madsen, S. et al. A simple filter-paper technique allows detection of mucosal cytokine levelsin vivo in ulcerative colitis. Digest Dis Sci 41, 1775–1779 (1996). https://doi.org/10.1007/BF02088744

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  • DOI: https://doi.org/10.1007/BF02088744

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