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Protective effect of nitric oxide on development of acute pancreatitis in rats

  • Pancreatic and Biliary Disorders
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Abstract

Nitric oxide (NO) has been implicated to regulate pancreatic circulation, promote capillary integrity, and inhibit leukocyte adhesion. We investigated the role of NO in the development of pancreatitis. Nitro-l-arginine, an inhibitor of NO synthase, in total dose of 35 mg/kg body wt was infused in the rats with edematous pancreatitis induced by two intraperitoneal injections of cerulein (20 μg/kg).l-Arginine (125 or 250 mg/kg), a NO donor was intravenously administered twice in the rats with hemorrhagic pancreatitis induced by waterimmersion stress plus two intraperitoneal injections of cerulein (40 μg/kg). The degree of pancreas edema, serum amylase levels, and histologic alterations were investigated. Nitro-l-arginine exacerbated cerulein-induced pancreatitis and caused a decrease in pancreatic blood flow.l-Arginine ameliorated the severity of hemorrhagic pancreatitis dose dependently and improved the pancreatic blood flow. These findings suggest that NO could confer protection against the development of hemorrhagic pancreatitis, probably through improvement of the pancreatic microcirculation.

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Authors and Affiliations

  1. Department of Internal Medicine, Faculty of Medicine, Kyushu University, 812-84, Fukuoka City, Japan

    Xiaohong Liu MD,  Itsuro Nakano MD, Hiroya Yamaguchi MD, Tetsuhide Ito MD, Masayoshi Goto MD, Shujiro Koyanagi MD, Mitsuru Kinjoh MD & Hajime Nawata MD

  2. Division of Clinical Pathology, Sanshinkai Hara Hospital, Fukuoka City, Japan

    Xiaohong Liu MD,  Itsuro Nakano MD, Hiroya Yamaguchi MD, Tetsuhide Ito MD, Masayoshi Goto MD, Shujiro Koyanagi MD, Mitsuru Kinjoh MD & Hajime Nawata MD

Authors
  1. Xiaohong Liu MD
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  2. Itsuro Nakano MD
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  3. Hiroya Yamaguchi MD
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  4. Tetsuhide Ito MD
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  5. Masayoshi Goto MD
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  6. Shujiro Koyanagi MD
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  7. Mitsuru Kinjoh MD
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  8. Hajime Nawata MD
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Additional information

This study was supported by the research fund of the “Research Committee on Intractable Diseases of the Pancreas” of the Japanese Ministry of Health and Welfare, and by a Grant-in-Aid for General Scientific Research from the Ministry of Education, Science and Culture, Japan.

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Liu, X., Nakano, I., Yamaguchi, H. et al. Protective effect of nitric oxide on development of acute pancreatitis in rats. Digest Dis Sci 40, 2162–2169 (1995). https://doi.org/10.1007/BF02209000

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  • DOI: https://doi.org/10.1007/BF02209000

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