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Detection of bacteria by surface-enhanced Raman spectroscopy

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Abstract

The detection and identification of dilute bacterial samples by surface-enhanced Raman spectroscopy has been explored by mixing aqueous suspensions of bacteria with a suspension of nanocolloidal silver particles. An estimate of the detection limit of E. coli was obtained by varying the concentration of bacteria. By correcting the Raman spectra for the broad librational OH band of water, reproducible spectra were obtained for E. coli concentrations as low as approximately 103 cfu/mL. To aid in the assignment of Raman bands, spectra for E. coli in D2O are also reported.

Light scattering apparatus used to detect bacteria

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Acknowledgements

The authors would like to thank Professors Daniel T. Schwartz and François Baneyx for their interest and advice. The contributions of Daniel Allred in Professor Schwartz’s laboratory and undergraduate researcher Tian Huang are highly appreciated. The authors would also like to thank the National Science Foundation for Grant Number CTS-9982413, the University of Washington/PNNL Joint Institute for Nanotechnology, and the University of Washington Center for Nanotechnology for financial support.

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Authors and Affiliations

  1. Department of Chemistry, University of Washington, Box 351700, Seattle, WA, 98195-1700, USA

    Atanu Sengupta

  2. Department of Chemical Engineering, University of Washington, Box 351750, Seattle, WA, 98195-1750, USA

    Mirna Mujacic & E. James Davis

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  1. Atanu Sengupta
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  2. Mirna Mujacic
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  3. E. James Davis
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Correspondence to Atanu Sengupta.

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Sengupta, A., Mujacic, M. & Davis, E.J. Detection of bacteria by surface-enhanced Raman spectroscopy. Anal Bioanal Chem 386, 1379–1386 (2006). https://doi.org/10.1007/s00216-006-0711-z

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  • DOI: https://doi.org/10.1007/s00216-006-0711-z

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