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Monoclonal antibody R5 for detection of putatively coeliac-toxic gliadin peptides

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An Erratum to this article was published on 21 February 2006

An Erratum to this article was published on 21 February 2006

An Erratum to this article was published on 21 February 2006

An Erratum to this article was published on 21 February 2006

Abstract

Monoclonal antibody R5 against rye secalin was recently suggested to be useful in analysis of gluten in food. The epitope specificity of R5 was characterized and compared with those of eight other monoclonal antibodies (mabs) against gliadins (gli) and secalins. Mabs were tested for binding to synthetic peptides spanning in overlapping manner sequences of gli. In a luminescence assay R5 bound to all peptides from the N-terminal part of α-type gli hitherto known to induce in sensitive patients with coeliac disease after in vivo instillation. Thus, R5 proves to be very useful for gluten analysis. Sequences QQPFP, QQQFP, LQPFP, and QLPFP were bound most strongly. Substitution of glutamine by glutamic acid in the epitope may decrease binding of R5 dependent on surrounding amino acids. One of the positions of the substitutions diminishing antibody binding was a typical site of attack of tissue transglutaminase, the enzyme converting by deamidation cereal prolamins into their disease active form. Investigation of eight other mabs against gli and secalins showed binding properties very similar to R5. We speculate the sequence QQQ/PFP seems to represent an immunodominant structure in prolamins.

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Acknowledgements

This work was supported by grants of Interdisciplinary Centre of Clinical Research (IZKF) of the University of Leipzig, 01KS9504, Project A5 (TM), of the German Academic Exchange Service, D/03/44431 and D/03/40308 (FK, TM) and AVCR D23-CZ/04-05 (DS, LT, HT), of the Saxon Ministry of Science and Art, 915-0504-WE (FK), of the Grant Agency of the Czech Republic, 310/04/P242 (DS), and of Plan Nacional AGL2004-02721/ALI, BIO2000-0403-P4-03, and PETRI 95-0778.OP (EM). Dr. J. Plicka (Immunotech, Prague) is thanked for help in preparing monoclonal antibodies 6H5, 8D4, and 5G7.

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Authors and Affiliations

  1. Institute of Laboratory Medicine, Clinical Chemistry, and Molecular Diagnostics, University Hospital Leipzig, Leipzig, Germany

    Franka Kahlenberg & Thomas Mothes

  2. Department of Immunology, Institute of Microbiology, Czech Academy of Sciences, Prague, Czech Republic

    Daniel Sanchez, Ludmila Tuckova & Helena Tlaskalova

  3. Roboscreen GmbH Leipzig, Leipzig, Germany

    Ingolf Lachmann

  4. Unidad de Gluten, Centro Nacional de Biotecnología, CSIC, Campus Universidad Autónoma, Cantoblanco, Madrid,, Spain

    Enrique Méndez

Authors
  1. Franka Kahlenberg
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  2. Daniel Sanchez
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  3. Ingolf Lachmann
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  4. Ludmila Tuckova
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  5. Helena Tlaskalova
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  6. Enrique Méndez
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  7. Thomas Mothes
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Correspondence to Thomas Mothes.

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An erratum to this article can be found at http://dx.doi.org/10.1007/s00217-006-0268-2

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Kahlenberg, F., Sanchez, D., Lachmann, I. et al. Monoclonal antibody R5 for detection of putatively coeliac-toxic gliadin peptides. Eur Food Res Technol 222, 78–82 (2006). https://doi.org/10.1007/s00217-005-0100-4

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  • DOI: https://doi.org/10.1007/s00217-005-0100-4

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