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High expression of Foxp3, IL-23p19 and survivin mRNA in colorectal carcinoma

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International Journal of Colorectal Disease Aims and scope Submit manuscript

Abstract

Introduction

Cytokines have been suggested to both modulate anti-tumor responses and promote tumor growth.

Materials and methods

We analyzed the expression of pro-inflammatory IL-12p35, IL-12p40, IL-23p19, anti-inflammatory IL-10, antiapoptotic factor survivin, and transcription factors—RelA, c-Jun, and Foxp3 mRNA in patients’ blood, colon carcinoma tissue, and in normal mucosal tissue by real-time polymerase chain reaction. The quantity determination of serum IL-12p40, IL-23, and IL-10 was performed by enzyme-linked immunosorbent assay.

Results

We observed significantly higher levels in patients for all three analyzed cytokines, with IL-23 concentration change being the highest. We detected the greatest upregulation of IL-23p19, Foxp3 and survivin mRNA in colorectal carcinomas than normal mucosa. A statistically significant upregulation of IL-12p40, IL-10, and c-Jun mRNA but not for IL-12p35 and RelA mRNA in tumor tissue comparing to normal tissue was also established.

Conclusions

In conclusion, we show a characteristic gene expression profile combining markers associated with inhibition of anti-tumor immune response (Foxp3, IL-10), inhibition of apoptosis (survivin), and induction of the cytokines with protumoral activity as IL-12p40 and IL-23p19 (IL-23) in the colorectal tumor tissue but not in peripheral blood of patients.

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Acknowledgment

This work was supported by Grant N: 4/2008 from the Fund for Scientific and Mobile project from Faculty of Medicine at the Trakia University-Stara Zagora, Bulgaria.

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Authors and Affiliations

  1. Department of Neurosurgery, Surgery and Urology, 2nd Surgery clinic, University Hospital, Faculty of Medicine, Trakia University, Stara Zagora, Bulgaria

    Noyko Stanilov

  2. Department of Molecular Biology, Immunology and Genetics, Faculty of Medicine, Trakia University, Armeiska 11 St., 6000, Stara Zagora, Bulgaria

    Lyuba Miteva, Nikolay Mintchev & Spaska Stanilova

Authors
  1. Noyko Stanilov
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  2. Lyuba Miteva
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  4. Spaska Stanilova
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Correspondence to Spaska Stanilova.

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Stanilov, N., Miteva, L., Mintchev, N. et al. High expression of Foxp3, IL-23p19 and survivin mRNA in colorectal carcinoma. Int J Colorectal Dis 24, 151–157 (2009). https://doi.org/10.1007/s00384-008-0588-8

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  • DOI: https://doi.org/10.1007/s00384-008-0588-8

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