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The human lysyl oxidase-like 2 protein functions as an amine oxidase toward collagen and elastin

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Abstract

The lysyl oxidase-like 2 (LOXL2) protein is a human paralogue of lysyl oxidase (LOX) that functions as an amine oxidase for formation of lysine-derived cross-links found in collagen and elastin. In addition to the C-terminal domains characteristic to the LOX family members, LOXL2 contains four scavenger receptor cysteine-rich (SRCR) domains in the N-terminus. In order to assess the amine oxidase activity of LOXL2, we expressed a series of recombinant LOXL2 proteins with deletions in the SRCR domains, using an Escherichia coli expression system. All of the purified recombinant LOXL2 proteins, with or without the SRCR domains in the N-terminus, showed significant amine oxidase activity toward several different types of collagen and elastin in in vitro amine oxidase assays, indicating deletion of the SRCR domains does not interfere with amine oxidase activity of LOXL2. Further, amine oxidase activity of LOXL2 was not susceptible to inhibition by β-aminopropionitrile, an irreversible inhibitor of LOX, suggesting a different enzymatic mechanism between these two paralogues.

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Acknowledgement

This study was supported by a grant of the Korean Health Technology R&D Project, Ministry for Health, Welfare & Family Affairs, Republic of Korea (grant number: A080041).

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Authors and Affiliations

  1. Department of Biochemistry, School of Medicine, Wonkwang University, Iksan, Jeonbuk, 570-749, Korea

    Young-Mi Kim & Youngho Kim

  2. Department of Oral and Maxillofacial Pathology, School of Dentistry, Wonkwang University, Iksan, Jeonbuk, 570-749, Korea

    Eun-Cheol Kim

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  1. Young-Mi Kim
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  2. Eun-Cheol Kim
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Correspondence to Youngho Kim.

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Young-Mi Kim, Eun-Cheol Kim equally contribute to this work.

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Kim, YM., Kim, EC. & Kim, Y. The human lysyl oxidase-like 2 protein functions as an amine oxidase toward collagen and elastin. Mol Biol Rep 38, 145–149 (2011). https://doi.org/10.1007/s11033-010-0088-0

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  • DOI: https://doi.org/10.1007/s11033-010-0088-0

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