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Overexpression of osteopontin in hepatocellular carcinoma and its relationships with metastasis, invasion of tumor cells

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Abstract

Osteopontin (OPN) plays an important role in metastasis and relapse of human cancer. However, the whole story of OPN relating to cancer has been far from clear untill now. To investigate the expression of OPN in hepatocellular carcinoma (HCC) and its relationships with recurrence and metastasis of HCC, normal and malignant liver tissues from patients with HCC were analyzed using immunohistochemical staining. OPN expression was inhibited by small interfering RNA (siRNA) in HCC cells lines, and then colony formation and matrigel invasion were examined. The results showed that expression of OPN was associated with metastasis of HCC with a positive rate of OPN in the tissue of HCC (70.00%), which was highly more obvious than those in paracarcinoma tissue and normal liver tissue (P < 0.01). In HCC cell lines, OPN depletion could reduce formed colony and metastasizing numbers in vitro. In conclusion, Expression of OPN in the tissue of HCC is related to metastasis or metastases. Specific siRNA could decrease expressions of OPN at both mRNA and protein levels, and abates the invasiveness of hepatocellular carcinoma cells, suggesting that OPN might be a promising agent for treatment of metastasis and recurrence of HCC.

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Authors and Affiliations

  1. Department of Hepatobiliary Surgery, First Municipal People’s Hospital of Guangzhou, The Affiliated Hospital of Guangzhou Medical College, Guangzhou, 510180, People’s Republic of China

    Fan Lin, YuYuan Li, Jie Cao, ShaoFeng Fan, JiFeng Wen, GuangHui Zhu, Hong Du & YanXiao Liang

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  1. Fan Lin
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  2. YuYuan Li
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  3. Jie Cao
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  6. GuangHui Zhu
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  7. Hong Du
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Correspondence to YuYuan Li.

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Lin, F., Li, Y., Cao, J. et al. Overexpression of osteopontin in hepatocellular carcinoma and its relationships with metastasis, invasion of tumor cells. Mol Biol Rep 38, 5205–5210 (2011). https://doi.org/10.1007/s11033-010-0671-4

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  • DOI: https://doi.org/10.1007/s11033-010-0671-4

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