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More Protection of Lactobacillus acidophilus Than Bifidobacterium bifidum Probiotics on Azoxymethane-Induced Mouse Colon Cancer

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Abstract

Based on the ability of the probiotics in the gut microflora modification, they can have the beneficial effects on diseases in the short and/or the long term. In previous study, we revealed that unlike Bifidobacterium bifidum, the amount of Lactobacillus acidophilus remained almost unchanged in mice gut microflora in the long term, indicating more stability of L. acidophilus than B. bifidum which can be used to prevent some incurable diseases such as cancer. Thirty-eight male BALB/c mice were divided into four groups, control, azoxymethane (AOM), L. acidophilus, and B. bifidum probiotics, to evaluate the protective effects of the probiotics on AOM-induced mouse colon cancer. Except for the control group, the rest of the animals were weekly given AOM (15 mg/kg, s.c) in three consecutive weeks. Colon lesion incidence was 74% in the AOM group in comparison with the control (0%) (P < 0.05). The lesions were varied from mild to severe dysplasia and colonic adenocarcinoma. Administration of the probiotics inhibited the incidence of colonic lesions by about 57% in L. acidophilus (P < 0.05) and 27% in B. bifidum (P > 0.05) compared to the AOM group. The serum levels of CEA and CA19-9 tumor markers were significantly decreased in L. acidophilus in comparison with the AOM group (P < 0.05). Moreover, the serum levels of IFN-γ and IL-10 and the number of CD4+ and CD8+ cells were significantly increased in L. acidophilus compared to AOM (P < 0.05). Our study highlighted the more potential effects of L. acidophilus probiotic than B. bifidum on mouse colon cancer.

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Acknowledgments

This study was supported by Tehran University of Medical Sciences (Grant Number: 17733) and Iran University of Medical Sciences (Grant Number: 18890).

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Authors and Affiliations

  1. Colorectal Research Center, Iran University of Medical Sciences, Tehran, Iran

    Shahram Agah

  2. Cancer Research Center, Tehran University of Medical Sciences, Tehran, 1419733141, Iran

    Ali Mohammad Alizadeh, Maryam Mosavi, Peyman Ranji & Hossein Khavari-Daneshvar

  3. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

    Farnaz Ghasemian

  4. Research and Development Department, Zist Takhmir Company, Tehran, Iran

    Sahar Bahmani

  5. Department of Pathology, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran

    Abbas Tavassoli

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  1. Shahram Agah
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  2. Ali Mohammad Alizadeh
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Correspondence to Ali Mohammad Alizadeh.

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All procedures performed in studies involving animals were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Declaration of Helsinki and its later amendments or comparable ethical standards.

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Agah, S., Alizadeh, A.M., Mosavi, M. et al. More Protection of Lactobacillus acidophilus Than Bifidobacterium bifidum Probiotics on Azoxymethane-Induced Mouse Colon Cancer. Probiotics & Antimicro. Prot. 11, 857–864 (2019). https://doi.org/10.1007/s12602-018-9425-8

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  • DOI: https://doi.org/10.1007/s12602-018-9425-8

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