Research paperQuantitation of plasma free cholesterol and cholesteryl esters by high performance liquid chromatography Study of a normal population
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2014, Journal of Steroid Biochemistry and Molecular BiologyCitation Excerpt :The coefficient of variation (CV) of these assays is <5%. Unesterified cholesterol and CE (cholesteryl linoleate, arachidonate, oleate, palmitate, and stearate) were measured by high performance liquid chromatography using a modification of the method described in [28]. CE were quantified relative to pure standards with cholesteryl heptadecanoate as internal standard.
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2002, AtherosclerosisCitation Excerpt :After evaporation of the organic solvent, the remainder was resolubilised with acetonitrile and isopropanol (1:1). The cholesterol and cholesterol ester contents of these solutions were measured by reverse-phase high-pressure liquid chromatography (RP-HPLC) using standard procedures as described by Vercaemst et al. [25]. The calibration procedure was necessary for each single compound, free cholesterol and each cholesteryl ester.
Estradiol esterification in the human preovulatory follicle
2001, SteroidsCitation Excerpt :To avoid contamination the column was washed, after each run, with 100% of methanol for 10 min and, then, 100% of the acetonitrile/water mixture for 10 min. Separation and quantitation of follicular fluid lin-C and free C was carried out essentially according to a published procedure [43]. In details, to 10 μl of sample 90 μl of water and, then, 200 μl of ethanol were added.