The repertoire of human germline vH sequences reveals about fifty groups of VH segments with different hypervariable loops

doi:10.1016/0022-2836(92)90223-7

Journal of Molecular Biology

Volume 227, Issue 3, 5 October 1992, Pages 776-798

https://doi.org/10.1016/0022-2836(92)90223-7 Get rights and content

Abstract

We have used the polymerase chain reaction and V_H family-based primers to clone and sequence 74 human germline V_H segments from a single individual and built a directory to include all known germline sequences. The directory contains 122 V_H segments with different nucleotide sequences, 83 of which have open reading frames. The directory indicates that the structural diversity of the germline repertoire for antigen binding is fixed by about 50 groups of V_H segments: each group encodes identical hypervariable loops. The directory should help in mapping the V_H locus, in estimating somatic mutation and V_H segment usage and in designing and constructing synthetic antibody libraries.

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Journal of Molecular Biology

ArticleThe repertoire of human germline vH sequences reveals about fifty groups of VH segments with different hypervariable loops

Abstract

Article
The repertoire of human germline v_H sequences reveals about fifty groups of V_H segments with different hypervariable loops