Separation and computerized gas chromatography-mass spectrometry of unconjugated neutral steroids in plasma

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Abstract

A method is described for analysis of unconjugated neutral steroids in plasma. It is based on extraction of the sample with Amberlite XAD-2 at elevated temperature, purification of the extract on lipophilic ion exchanging and neutral Sephadex derivatives, and gas chromatography-mass spectrometry-computer analysis of O-methyloxime-trimethylsilyl ether derivatives purified on hydrophobic Sephadex. Examples of the application of the method to analysis of steroids in plasma from pregnant women are given.

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