Cell
Volume 76, Issue 6, 25 March 1994, Pages 1063-1073
Journal home page for Cell

Article
Location of stem cells of human hair follicles by clonal analysis

https://doi.org/10.1016/0092-8674(94)90383-2Get rights and content

Abstract

We have examined the growth capacity of keratinocytes isolated from human scalp hair follicles. Like the keratinocytes of glabrous epidermis, most of the colony-forming cells are classified as holoclones or meroclones when analyzed in a clonal assay. Some of them have extensive growth potential, as they are able to undergo at least 130 doublings. Therefore, the hair follicle, like the epidermis, contains keratinocytes with the expected property of stem cells: an extensive proliferative capacity permitting the generation of a large amount of epithelium. We have also examined the distribution of clonogenic keratinocytes within the hair follicle. Several hundred colony-forming cells are concentrated at a region below the midpoint of the follicle and outside the hair bulb. This region lies deeper than the site of insertion of the arrector pili muscle, which corresponds with the position of the bulge when the latter can be identified. In contrast, few colony-forming cells are present in the hair bulb, where most of the mitotic activity is observed during the active growth phase of the follicle. Paraclones, which are present both in the midregion and in the bulb of hair follicles, are unlikely to be the transient amplifying cells expected from kinetic studies.

References (44)

  • H. Pinkus

    Embryology of hair

  • A.J. Reynolds et al.

    Human hair follicle germinative epidermal cell culture

    J. Invest. Dermatol.

    (1993)
  • M. Simon et al.

    Enzymatic cross-linking of involucrin and other proteins by keratinocyte particulates in vitro

    Cell

    (1985)
  • E.J. Van Scott et al.

    Determinants of rate and kinetics of cell division in scalp hair

    J. Invest. Dermatol.

    (1963)
  • G.D. Weinstein et al.

    Cell proliferation in normal epidermis

    J. Invest. Dermatol.

    (1984)
  • J.-S. Yang et al.

    Upper human hair follicle contains a subpopulation of keratinocytes with superior in vitro proliferative potential

    J. Invest. Dermatol.

    (1993)
  • S.E. Al-Barwari et al.

    Regeneration and dose-response characteristics of irradiated mouse dorsal epidermal cells

    Int. J. Radiat. Biol.

    (1976)
  • B.L. Allen-Hoffmann et al.

    Polycyclic aromatic hydrocarbon mutagenesis of human epidermal keratinocytes in culture

  • Y. Barrandon et al.

    Cell size as a determinant of the clone-forming ability of human keratinocytes

  • Y. Barrandon et al.

    Three clonal types of keratinocyte with different capacities for multiplication

  • H.M. Blau et al.

    Plasticity of the differentiated state

    Science

    (1985)
  • H.B. Chase

    Growth of the hair

    Physiol. Rev.

    (1954)
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    Present address: Biological Laboratory, College of Arts and Sciences, Chiba University, 1–33 Yayoi-cho, Inage-ku, Chiba, 263, Japan

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