Evaluation of growth rate in adhering cell cultures using a simple colorimetric method
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2017, MitochondrionCitation Excerpt :Cell growth and mitochondrial function were assessed after a 72-hour incubation period. Cell growth was assessed by measuring cellular content with a colorimetric method based on methylene blue (MB) staining of basophilic cellular components, which is independent of redox status (Pelletier et al., 1988). Cells in the microtiter wells were fixed with glutaraldehyde and stained with 1% MB in borate buffer, before being rinsed and dried.
Development and characterization of a new gill cell line from air breathing fish Channa striatus (Bloch 1793) and its application in toxicology: Direct comparison to the acute fish toxicity
2014, ChemosphereCitation Excerpt :Fluorescence was expressed as a percentage of control (cells with media alone) after reading the subtraction of background fluorescence (blank without cells). Total cell protein content was measured following the procedure described by Pelletier et al. (1988), using the Methylene Blue dye. After 24 h exposure, the medium was removed and each well washed twice with 200 μL of PBS.
The effect of small molecules on nuclear-encoded translation diseases
2014, BiochimieCitation Excerpt :Subsequently after 72 h, cell growth, reactive oxygen species production, ATP content, mitochondrial content, mitochondrial membrane potential and enzymatic activities were assayed in microtiter plates as described below. Cell growth was measured by a colorimetric method using methylene blue (MB) based on the staining of basophilic cellular components (mainly nucleic acids), basically as described [31]. Cells were fixed with 0.5% gluteraldehyde for 10 min, rinsed with double-distilled water, stained with 1% methylene blue in 0.1 M borate buffer for 1 h, rinsed with water and allowed to dry.
Altered fibroblast function following myocardial infarction
2005, Journal of Molecular and Cellular CardiologyCitation Excerpt :The plates were stained with 1% methylene blue. After eluting stain with acid alcohol (0.05 M HCl in 50% ethanol), the plates were read at an absorbance of 620 nm [20–22]. Cell counts were also taken at first passage and normalized to the weight of the tissue to obtain an additional index of proliferation.