Article
Somatostatin antibody does not influence bombesin-induced inhibition of gastric acid secretion in rats

https://doi.org/10.1016/0196-9781(94)00152-VGet rights and content

Abstract

The influence of peripheral somatostatin immunoneutralization on intravenous (IV) and intracerebroventricular (ICV) bombesin-induced inhibition of gastric acid secretion (GAS) was investigated with the somatostatin monoclonal antibody, CURE.S6, in rats. The somatostatin antibody, injected IV in conscious rats with a chronic gastric fistula and IV catheter, did not modify basal GAS, whereas in urethane-anesthetized rats the basal GAS was increased by 150%. In conscious rats, somatostatin (15 μg/kg/h, IV) inhibited basal GAS by 50% after injection of control antibody but not after pretreatment with the somatostatin antibody. With pretreatment with a control antibody, bombesin (10 μg/kg/h, IV) inhibited basal GAS by 60% in conscious rats and by 50% the acid response to pentagastrin infusion in urethane-anesthetized rats. Bombesin injected ICV (3 and 10 ng/10 μl/rat) inhibited basal GAS by 50% and 70%, respectively, in conscious rats pretreated with a control antibody. The somatostatin antibody injected IV before IV or ICV injection of bombesin did not influence bombesin-induced inhibition of GAS in conscious or anesthetized rats. These results show that peripheral somatostatin does not play a major role in the inhibition of gastric acid secretion induced either by ICV or IV administration of bombesin or basal acid secretion in conscious rats.

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