Cancer Letters

Cancer Letters

Volume 53, Issue 1, August 1990, Pages 17-25
Cancer Letters

Inhibition of the promotional phase of azoxymethane-induced colon carcinogenesis in the F344 rat by calcium lactate: effect of simulating two human nutrient density levels

https://doi.org/10.1016/0304-3835(90)90005-IGet rights and content

Abstract

The effects of 2 levels of dietary calcium and 2 types of dietary fat on the promotional phase phase of azoxymethane-induced colon cancer in the F344 rat were investigated. During the initiation phase of carcinogenesis all animals were fed a 5% corn oil AIN-76A diet containing 0.32% Ca in the form of calcium lactate. Rats were then injected with azoxymethane (AOM) weekly for 8 weeks. Thereafter, the rats were fed 1 of 3 diet formulations: a 5% corn oil diet or a 20% corn oil or 20% American Blend oil fat diet, with the level of Ca set at either 0.32% of the diet, a nutrient density simulating a daily human intake of ∼ 1700 mg Ca/day, or at 0.04% of the diet, reflecting a human daily intake of ∼ 200–250 mg of Ca/day, thus modeling 2 human nutrient density levels for calcium. Measurements of fecal pH during the experiment indicated an acidic adaptation of the large bowel to the lactate anion. Analysis of collected fecal samples showed more total fatty acids to be present in the colon when higher amounts of calcium were consumed. However, results of the tumorigenesis study indicated that calcium lactate fed at the 0.32% level significantly inhibited the development of colonic adenocarcinoma in all dietary groups. Taken together, this investigation supports the hypothesis that calcium supplementation can inhibit colon neoplasia in rats fed a high fat diet; however, under the conditions of this study, the 20% fat level did not significantly promote colon cancer as compared to a 5% fat level.

References (30)

  • F.R. Steggerda et al.

    The calcium balance of adult human subjects on high and low butter diets

    J. Nutr.

    (1951)
  • R.P. Bird

    Effect of dietary components on the pathobiology of colon epithelium: possible relationship with colon tumorigenesis

    Lipids

    (1986)
  • K.K. Carroll

    Experimental studies on dietary fat and cancer in relationship to epidemiological data

  • W.V. Dokkum et al.

    Diet, faecal pH and colorectal cancer

    Br. J. Cancer

    (1983)
  • W. Esteves et al.

    Determination of lipids, including medium-chain fatty acids in human feces

    Clin. Chem.

    (1982)
  • D.Y. Graham et al.

    Solubility of calcium soaps of short chain fatty acids in simulated intestinal environment

    Digest. Dis. Sci.

    (1983)
  • G. Isbell et al.

    Calcium modulation of mucosal proliferation in patients with sporadic adenoma or colon cancer

    Gastroenterology

    (1989)
  • E.A. Jacobson et al.

    Effects of dietary fat, calcium and vitamin D and mammary tumorigenesis induced by 7,12-dimethylbenz(a)anthracene in female Sprague-Dawley rats

    Cancer Res.

    (1989)
  • H.H. LeVeen et al.

    Cause and treatment of diarrhea following reaction of the small intestine

    Surg. Gynecol. Oncol.

    (1967)
  • N. Lipkin

    Biomarkers of increased susceptibility: their application to studies of cancer prevention

    Gastroenterology

    (1987)
  • N. Lipkin

    Biomarkers of increased susceptibility to gastrointestinal cancer: new application to studies of cancer prevention in human subjects

    Cancer Res

    (1988)
  • N. Lipkin et al.

    Colonic epithelial cell proliferation in responders and nonresponders to supplemental dietary calcium

    Cancer Res.

    (1989)
  • N. Lipkin et al.

    Effect of added dietary calcium on colonic epithelial cell proliferation in subjects at high risk for familial colon cancer

    N. Engl. J. Med.

    (1985)
  • H.L. Newmark

    Nutrient density: an important and useful tool for laboratory animal studies

    Cancinogenesis

    (1987)
  • H.L. Newmark et al.

    Colon cancer and dietary fat, calcium and phosphate: a hypothesis

    J. Natl. Cancer Inst.

    (1984)
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