Rapid CommunicationChemoprevention of spontaneous intestinal adenomas in the adenomatous polyposis coli Min mouse model with aspirin☆,☆☆
Section snippets
Animals
Nine male and 9 female C57BL/6J +/+ (control) mice and 9 male and 9 female C57BL/6J Min mice were purchased from Jackson Laboratories (Bar Harbor, ME) at 5 weeks of age. Upon receipt, male Min mice weighed significantly less than male control mice (22.4 ± 0.3 vs. 23.6 ± 0.3 g, mean ± SEM, respectively; P = 0.02), but there was no significant difference in initial body weight between Min and control females (17.3 ± 0.2 vs. 18.3 ± 0.4 g, respectively; P = 0.07). Mice had free access to food and
Results
At the end of 7 weeks, all Min mice had developed spontaneous intestinal tumors, whereas none of the control C57BL/6J mice had tumors. Histological examination of H&E-stained tumor sections showed that two small intestinal tumors were locally invasive through the muscularis mucosa, whereas the remaining tumors were adenomas.
As shown in Table 1, aspirin added to the diet at both 250 and 500 ppm significantly (P < 0.05) reduced tumor multiplicity (number of tumors per mouse) in the proximal,
Discussion
Human epidemiological data and experiments utilizing carcinogen-induced rodent models of colon cancer have provided ample evidence indicating that aspirin is an important colon cancer chemopreventive agent.1 Our data show for the first time that aspirin reduces the formation of spontaneous intestinal tumors in mice with a genetic predisposition to develop intestinal tumors, thus providing additional evidence for regular aspirin use as an effective chemopreventive strategy. In the present study,
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2021, Life Sciences in Space ResearchCitation Excerpt :Since the duration of aspirin use for effective GI-cancer prevention is also not established in mouse or human studies (Bosetti et al., 2020) therefore further studies might be required to optimize aspirin timing and duration to achieve desired chemoprevention. In another Apc-based GI-cancer model i.e. ApcMin/+ mice up to 50% chemoprevention was achieved but with a bolus aspirin dose (Barnes & Lee, 1998), which is many-fold higher than the adult human dose. However, in ApcMin/+ mice it was noted that in-utero aspirin use is more effective compared to aspirin use after weaning (Perkins et al., 2003; Rohwer et al., 2020).
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Address requests for reprints to: Makau Lee, M.D., Ph.D, Department of Medicine/Division of Gastroenterology, University of Texas Health Science Center, 7703 Floyd Curl Drive, San Antonio, Texas 78284-7878. e-mail: [email protected]; fax: (210) 567-1976.
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Supported in part by the San Antonio Cancer Institute and by National Cancer Institute Research Center Award P30 CA 54174. Makau Lee is a recipient of the Paul Beeson Physician Faculty Scholars in Aging Research Award.