Liver, Pancreas, and Biliary TractEffect of bacterial or porcine lipase with low- or high-fat diets on nutrient absorption in pancreatic-insufficient dogs☆,☆☆
Section snippets
Preparation of pancreatic-insufficient dogs and their maintenance
All procedures and experiments were reviewed and approved by the Institutional Animal Care and Use Committee of the Mayo Foundation in accordance with the guidelines of the National Institutes of Health and the Public Health Policy on the Humane Use and Care of Laboratory Animals.
Five female dogs weighing between 18 and 21 kg were used. Dogs were anesthetized with an intravenous injection of thiopental sodium and then underwent endotracheal intubation. Anesthesia was maintained by halothane
Effects of exocrine pancreatic insufficiency on body weight, food intake, and blood glucose
Dogs with exocrine pancreatic insufficiency lost 7% ± 2% (2%–13%) of their body weight, but they continued to rapidly ingest entire meals. There were no significant changes in postprandial blood glucose levels during the studies (data not shown).
Dose response and comparison of the effects on steatorrhea between BL and PL with maintenance diet (27% fat)
After operation, untreated pancreatic-insufficient dogs absorbed less fat (CFA, 63% ± 6% vs. 96% ± 1%; P < 0.01; Figure 1).
Discussion
Human pancreatic steatorrhea is rarely eliminated by current therapy. In the present and a previous study,1 we report that combining 300,000 IU of BL with a high proportion of fat in the diet corrects steatorrhea in canine exocrine insufficiency. These data engender hope that BL and diet therapy may improve treatment of pancreatic steatorrhea. Now we also show that a high-fat and high-protein diet improves CFA in response to PL. Thus, we suggest that in humans with pancreatic steatorrhea,
References (28)
- et al.
Bacterial lipase and high-fat diets in canine exocrine pancreatic insufficiency: a new therapy of steatorrhea?
Gastroenterology
(1997) - et al.
Cr ystallization and preliminary x-ray study of a lipase from Pseudomonas glumae
J Mol Biol
(1992) - et al.
Lipolytic activity of bacterial lipase survives better than that of porcine lipase in human gastric and duodenal content
Gastroenterology
(1994) - et al.
How to protect human pancreatic enzyme activities in frozen duodenal juice
Gastroenterology
(1991) - et al.
Rapid method for the determination of fat in feces
J Biol Chem
(1949) - et al.
Effects of decreasing intraluminal amylase activity on starch digestion and postprandial gastrointestinal function in humans
Gastroenterology
(1986) - et al.
Comparative evaluation of the therapeutic efficacy of a pH-sensitive enteric-coated pancreatic enzyme preparation with conventional pancreatic enzyme therapy in the treatment of exocrine pancreatic insufficiency
Gastroenter-ology
(1983) - et al.
Strictures of ascending colon in cystic fibrosis and high-strength pancreatic enzymes
Lancet
(1994) Colonic strictures in cystic fibrosis
Lancet
(1994)- et al.
Improved respiratory prognosis in cystic fibrosis patients with normal fat absorption
J Pediatr
(1982)
The different courses of early- and late-onset idiopathic and alcoholic chronic pancreatitis
Gastroenterology
Therapeutic potential and clinical efficacy of acid-resistant fungal lipase in the treatment of pancreatic steatorrhea due to cystic fibrosis
Pancreas
Protection of lipolytic activity (L) by nutrients in simulated pancreatic insufficiency
Pancreas
Stability of pancreatic enzyme activities in duodenal juice after pancreatic stimulation by a test meal or exogenous hormones
Ann Clin Biochem
Cited by (0)
- ☆
Address requests for reprints to: Eugene P. DiMagno, M.D., Gastroenterology Research Unit (Alfred 2-435), Mayo Clinic, 200 First Street Southwest, Rochester, Minnesota 55905. Fax: (507) 255-6318.
- ☆☆
Supported in part by a grant from Knoll AG, Ludwigshafen, Germany, and the Mayo Foundation.