The joys of in vitro selection: chemically dressing oligonucleotides to satiate protein targets
References (65)
- et al.
Searching for strategies for applied molecular evolution
J Theor Biol
(1995) - et al.
Artificial evolution and natural ribozymes
FASEB J
(1995) - et al.
Porphyrin metalation catalyzed by a small RNA molecule
J Am Chem Soc
(1996) - et al.
Calcium-dependent oligonucleotide antagonists specific for L-selectin
Proc Natl Acad Sci USA
(1996) - et al.
High-affinity RNA ligands to Escherichia coli ribosomes and ribosomal protein S1: comparison of natural and unnatural binding sites
Biochemistry
(1995) - et al.
New uridine derivatives for use in systematic evolution of RNA ligands by exponential enrichment
J Am Chem Soc
(1995) - et al.
Novel nucleosides via intramolecular functionalization of 2,2′-anhydrouridine derivatives
Tetrahedron Lett
(1996) The rise and fall of the RNA world
New Biol
(1991)- et al.
Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase
Science
(1990) - et al.
In vitro selection of RNA molecules that bind specific ligands
Nature
(1990)
Adaptive walks with noisy fitness measurements
Mol Divers
Diversity of oligonucleotide functions
Annu Rev Biochem
In vitro selection of aptamers: the dearth of pure reason
Curr Biol
Oligonucleotides as research, diagnostic, and therapeutic agents
J Biol Chem
Chance and necessity in the selection of nucleic acid catalysts
Accounts Chem Res
Ribozyme-catalysed amino-acid transfer reactions
Nature
Aminoacyl-RNA synthesis catalyzed by an RNA
Science
Specialization of the DNA-cleaving activity of a Group I ribozyme through in vitro evolution
J Mol Biol
In vitro evolution of a self-alkylating ribozyme
Nature
In vitro selection of a novel catalytic RNA: characterization of a sulfur alkylation reaction and interaction with a small peptide
RNA
Structurally complex and highly active RNA ligases derived from random RNA sequences
Science
A DNA metalloenzyme with DNA ligase activity
Nature
Synthetic ribozymes and the first deoxyribozyme
Angew Chem I Int Ed
Kinetic and mechanistic analysis of nonenzymatic, template-directed oligonucleotide ligation
J Am Chem Soc
RNA-catalyzed RNA polymerization using nucleoside triphosphates
Nature
Nonenzymatic, template-directed ligation of oligonucleotides is a highly regioselective for the formation of 3′→5′ phosphosphodiester bonds
J Am Chem Soc
RNA molecules that specifically and stoichiometrically bind aminoglycoside antibiotics with high affinities
Biochemistry
A novel RNA motif for neomycin recognition
Chem Biol
Specific binding of aminoglycoside antibiotics to RNA
Chem Biol
Structural probing and damage selection of citrulline- and arginine-specific RNA aptamers identify base positions required for binding
Nucleic Acids Res
A DNA aptamer that binds adenosine and ATP
Biochemistry
RNA aptamers that bind flavin and nicotinamide redox cofactors
J Am Chem Soc
Cited by (98)
An efficient method to evaluate experimental factor influence on in vitro binding of aptamers
2018, Analytical BiochemistryCitation Excerpt :The binding step is also critical to minimize the nonspecific binding in the selected pool. Numerous factors can have an impact on the nonspecific binding, such as the properties of the aptamers (including nucleic acid type, length, base composition and modification) [37–39], the target, as well as the experimental factors (including ionic strength, buffering agent, temperature, pH, and so on) [40]. The situation is even more complicated when the aptamer selection is performed in complex matrix, for example, buffer containing a cocktail of proteins and other metabolites similar to the condition under which the aptamer would be expected to work in.
Biophysics and protein corona analysis of Janus cyclodextrin-DNA nanocomplexes. Efficient cellular transfection on cancer cells
2017, Biochimica et Biophysica Acta - General SubjectsCitation Excerpt :However, in the case of biomolecular drugs, such as nucleic acids, the carrier becomes a decisive protagonist. In the free state, nucleic acids are easily degraded by nucleases in biological fluids [13,14], and their membrane-crossing abilities and cellular uptake are seriously limited by their negative charge, inherently large size, and rigidity [15]. Formulation with appropriate delivery systems is thus essential for nucleic acids to overcome the physiological barriers, reach the target in a fully functional form and carry out the designed therapeutic function.
FDA-Approved Oligonucleotide Therapies in 2017
2017, Molecular TherapyCitation Excerpt :Macugen (formerly pegaptanib) is an aptamer targeted to vascular endothelial growth factor (VEGF165). The molecule was first produced by the systematic evolution of ligands by exponential enrichment (SELEX)2,3 strategy pioneered by Larry Gold (an excellent review by Ng et al.4 provides a brief, concise description of the SELEX approach). Pegaptanib is 27 nucleobases in length.
Imidazole-free purification of His<inf>3</inf>-tagged recombinant proteins using ssDNA aptamer-based affinity chromatography
2015, Journal of Chromatography ACitation Excerpt :The aptamers may have a wide range of applications, including molecular biology, biotechnology and medicine [19]. In the last 20 years, many examples of aptamers developed against different targets, including proteins and even cells have been reported [20–23]. The dynamic development of research on ssDNA aptamers is stimulated by their features, e.g. binding strength comparable to antibodies [24], low immunogenicity [23], the simple, fast and relatively cheap synthesis.
9.02 - Lifelike but Not Living: Selection of Synthetically Modified Bioinspired Nucleic Acids for Binding and Catalysis
2012, Polymer Science: a Comprehensive Reference: Volume 1-10