Differentiation of human adipose stromal cells into hepatic lineage in vitro and in vivo
Section snippets
Isolation and culture of hADSC and HepG2 cell line
hADSC culture. After informed consent, leftover adipose tissues were obtained from four different donors (19 years old; male, 28 years old; female, 36 years old; female, and 55 years old; male) undergoing elective abdominoplasty. To isolate hADSC, adipose tissues were washed with equal volumes of phosphate-buffered saline (PBS), and tissues were digested at 37 °C for 30 min with 0.075% collagenase type I (Sigma). Enzyme activity was neutralized with α-modified Eagle’s medium (α-MEM), containing
Morphologic changes in cultured hADSC treated with HGF, OSM or DMSO
To specify antigenic properties of hADSC, we determined surface protein expression by flow cytometry. hADSC expressed CD29, CD44, CD105, and CD90, but did not express CD34, CD45, CD14, and HLA-DR, as described in our previous study [26]. To determine optimal conditions for hADSC differentiation into hepatocyte-like cells, we tested the effect of different cytokines on morphological changes of hADSC in the fibronectin-coated dishes. Differentiation was induced at 90% confluency of cells after
Discussion
MSC could differentiate into cells of all mesodermal origin, including adipocytes, osteocytes, chondrocytes, myocytes, and endothelial cells [20], [21], [22], [23]. Besides these, MSC are also capable of “transdifferentiation” into ectodermal cells, such as neural cells [27], [28]. These findings suggest that MSC belong to multipotent adult stem cells. Schwartz et al. [11] isolated a non-hematopoietic stem cell subset (CD45−GlyA− in humans or CD45−Ter119− in mice) from bone marrow, termed
Acknowledgment
This work was supported by a grant (02-PJ10-PG8-EC01-0018) from the Ministry of Health and Welfare.
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