Cell Reports
Volume 7, Issue 3, 8 May 2014, Pages 645-653
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Isolation and Molecular Characterization of Circulating Melanoma Cells

https://doi.org/10.1016/j.celrep.2014.03.039Get rights and content
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Highlights

  • Microfluidic isolation of circulating melanoma cells in a B-RAF-driven mouse model

  • CTC numbers correlate with tumor response to B-RAF-targeted therapies

  • CTCs are shed early and postresection B-RAF inhibition prevents metastasis in mouse

  • RNA-seq of mouse CTCs identifies a signature related to melanoma invasiveness

Summary

Melanoma is an invasive malignancy with a high frequency of blood-borne metastases, but circulating tumor cells (CTCs) have not been readily isolated. We adapted microfluidic CTC capture to a tamoxifen-driven B-RAF/PTEN mouse melanoma model. CTCs were detected in all tumor-bearing mice and rapidly declined after B-RAF inhibitor treatment. CTCs were shed early from localized tumors, and a short course of B-RAF inhibition following surgical resection was sufficient to dramatically suppress distant metastases. The large number of CTCs in melanoma-bearing mice enabled a comparison of RNA-sequencing profiles with matched primary tumors. A mouse melanoma CTC-derived signature correlated with invasiveness and cellular motility in human melanoma. CTCs were detected in smaller numbers in patients with metastatic melanoma and declined with successful B-RAF-targeted therapy. Together, the capture and molecular characterization of CTCs provide insight into the hematogenous spread of melanoma.

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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).