Clinical InvestigationSynchrony of telomere length among hematopoietic cells
Section snippets
Subjects
UCB was obtained from the placenta of eight full-term normal newborns (six males, two females); one African American; six whites of Hispanic extraction; and one of mixed extraction.
UCB processing
UCB in citric acid/dextrose was obtained from The Southwest Blood and Tissue Center, San Antonio, TX. One milliliter of blood was spun down for 10 minutes at 1,500g in a cooled centrifuge and the Buffy coat layer was collected and saved at −80°C.
Ficoll separation of mononuclear cells
For Ficoll separation, blood was diluted 1:1 in RPMI-1640 medium
First phase
Figure 1A displays the relationships of telomere length in memory and naïve CD4+ and CD8+ T cells with CD34+ cells, which are primarily HPCs. There were strong correlations of telomere length in all T-cell subsets with telomere length in CD34+ cells. Using paired t-test, telomere length was 190 bp shorter in CD4+ memory cells than in CD4+ naïve cells (Fig. 2A) and 150 bp shorter in CD8+ memory than CD8+ naïve cells (Fig. 2B). In addition, there were strong correlations of telomere length
Discussion
The main findings of the study are as follows: at birth, telomere length in T cells and granulocytes are strongly correlated with telomere length in HPCs; in both newborns and adults, telomere length in granulocytes is highly correlated with LTL. These findings extend the concept of synchrony (equivalence) observed within tissues and organs of the newborn [20] and the fetus [22] to HPCs and leukocyte subsets in the newborn. They also show that adults with long (or short) LTL display long (or
Acknowledgments
Research reported in this article was supported by National Heart, Lung and Blood Institute (Bethesda, MD, USA) grants R01AG21593 and R01AG20132 and grants from the Canadian Institutes of Health Research (Ottawa, ON, Canada) (MOP38075 and GMH79042) and the National Cancer Institute of Canada (with support from the Terry Fox Run; Vancouver, BC, Canada).
Conflict of Interest Disclosure
There are no financial conflicts of interest for any of the authors.
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