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Duck hepatitis B virus: a model to assess efficacy of disinfectants against hepadnavirus infectivity

Published online by Cambridge University Press:  19 October 2009

S. M. Murray ,
J. S. Freiman ,
K. Vickery ,
D. Lim ,
Y. E. Cossart  and
R. K. Whiteley
S. M. Murray
Affiliation:
Department of Infectious Diseases, University of Sydney, NSW, Australia, 2006
J. S. Freiman
Affiliation:
Department of Infectious Diseases, University of Sydney, NSW, Australia, 2006
K. Vickery*
Affiliation:
Department of Infectious Diseases, University of Sydney, NSW, Australia, 2006
D. Lim
Affiliation:
Department of Infectious Diseases, University of Sydney, NSW, Australia, 2006
Y. E. Cossart
Affiliation:
Department of Infectious Diseases, University of Sydney, NSW, Australia, 2006
R. K. Whiteley
Affiliation:
Department of Infectious Diseases, University of Sydney, NSW, Australia, 2006
*
*K. Vickery, Department of Infections Diseases, University of Sydney, Sydney, NSW 2006, Australia.
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Summary

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The efficacy of three proprietary glutaraldehyde disinfectants and their component bases was assessed using the duck hepatitis B virus (DHBV) model. Inactivation of infectivity of undiluted serum containing 106·8 ID50/ml DHBV was assessed after a mixture with an equal volume of disinfectant had stood at room temperature for 10 min. A dried spill of infectious serum was simulated using sterile filter paper disks, saturated with serum containing DHBV, dried and then exposed to test disinfectant for 10 min. Residual infectivity, and hence the reduction in virus titre, was determined by inoculation of dilutions of the treated samples into 1-day-old ducklings. A greater than 3 log10 reduction in virus titre could be demonstrated for the disinfectants as well as for some of their component bases. Disinfectant activity varied according to the method of viral presentation but a reduction of exposure time from 10 to 2·5 min did not diminish activity. The experimental protocol permits a comparative and quantitative assessment of the efficacy of both established and new disinfectants.

Type
Research Article
Information
Epidemiology & Infection , Volume 106 , Issue 3 , June 1991 , pp. 435 - 443
Copyright
Copyright © Cambridge University Press 1991

References

REFERENCES

1.Braconier, JH, Nordenfelt, E.Serum hepatitis in a home for the aged. J Infect Dis 1972; 4: 79.Google Scholar
2.Pattison, CP, Boyer, KM, Maynard, JE, Kelly, PC.Epidemic hepatitis in a clinical laboratory. J Amer Med Ass 1974; 230: 854–79.CrossRefGoogle Scholar
3.Bond, WW, Moncada, RE.Viral hepatitis B infection risk in flexible fibreoptic endoscopy. Gastrointest Endosc 1978; 24: 225–30.CrossRefGoogle Scholar
4.Marion, PL, Robinson, WS.Hepadna viruses: hepatitis B and related viruses. Current Topics Microbiol Immunol 1983; 105: 99–121.Google ScholarPubMed
5.Summers, J, Mason, WS.Replication of the genome of a hepatitis B-like virus by reverse transcriptase of an RNA intermediate. Cell 1982; 29: 403–15.CrossRefGoogle ScholarPubMed
6.Sprengel, R, Will, H. Duck hepatitis B virus. In: Y, Becker, Darae, G, eds. Virus disease in laboratory and captive animals. Martinus Nijohff Pub 1987: 9.Google Scholar
7.Jilbert, AR, Freiman, JS, Burrell, CJ et al. Early virus cell interactions in duck hepatitis B virus infection: a time-course study of tissue tropism and sites of virus production. In: AJ, Zuckerman, ed. Viral hepatitis and liver disease. New York: Alan RLiss Inc., 1988; 497–500.Google Scholar
8.Marion, PL, Knight, SS, Ho, BK, Guo, YY, Robinson, WS, Popper, H.Liver disease associated with duck hepatitis B virus infection of domestic ducks. Proc Nat Acad Sci (USA) 1984; 81: 889–92.CrossRefGoogle ScholarPubMed
9.Omata, M, Uchiumi, K, Ito, Y et al. Duck hepatitis B virus and liver disease. Gastroenterol 1983; 85: 260–7.CrossRefGoogle Scholar
10.Kobayashi, H, Tsuzuki, M, Koshimizu, K et al. Susceptibility of hepatitis B virus to disinfectants or heat. J Clin Microbiol 1984; 20: 214–6.CrossRefGoogle ScholarPubMed
11.Bond, WW, Favero, MS, Petersen, NJ, Ebert, JW.Inactivation of hepatitis B virus by intermediate-to-high-level disinfectant chemicals. J Clin Microbiol 1983; 18: 535–8.CrossRefGoogle ScholarPubMed
12.Marion, PL.Uses of animal models to study hepatitis B virus. Prog Med Virol 1988; 35: 43–75.Google Scholar
13.Weller, IVD, Williams, CB, Jeffrey, DJ et al. Cleaning and disinfection equipment for gastrointestinal flexible endoscopy: interim recommendations of a Working Party of the British Society of Gastroenterology. Gut 1988; 29: 1134–51.Google Scholar
14.Russell, AD, Hopwood, D. The biological uses and importance of glutaraldehyde. In: GP, Ellis, GB, West, eds. Progress in medicinal chemistry. North-Holland Publishing Company, 1976; 13: 271–99.Google Scholar
15.Scott, EM, Gorman, SP. Sterilization with glutaraldehyde. In: SS, Block, ed. Disinfection, sterilization and preservation. 3rd ed.Philadelphia: Lea and Febiger, 1983; 981–97.Google Scholar
16.Buscher, M, Reiser, W, Will, H, Schaller, H.Transcripts and the putative RNA pregenome of duck hepatitis B virus implications for reverse transcription. Cell 1985; 40: 717–24.CrossRefGoogle ScholarPubMed
17.Reed, LJ, Muench, H.A simple method estimating fifty percent end-points. Amer J Hyg 1938; 27: 493–7.Google Scholar
18.Mason, WS, Aldrich, C, Summers, J, Taylor, JM.Asymmetric replication of duck hepatitis B virus DNA in liver cells: free minus-strand DNA. Procs Nat Acad Sci (USA) 1982; 72: 3997–4001.CrossRefGoogle Scholar
19.Freiman, JS, Cossart, YE.Natural hepatitis B virus infection in Australia. Aust J Exp Biol Med Sci 1986; 64: 477–84.CrossRefGoogle ScholarPubMed
20.Freiman, JS, Jilbert, AR, Dixon, RJ et al. Experimental duck hepatitis infection: pathology and evolution of hepatic and extrahepatic infection. Hepatology 1988; 8: 507–13.CrossRefGoogle ScholarPubMed
21.Howard, CR, Dixon, J, Young, P et al. Chemical inactivation of HBV: the effect of disinfectants on virus-associated DNA polymerase activity, morphology and infectivity. J Virol Methods 1983; 7: 135–48.CrossRefGoogle ScholarPubMed
22.Zhao, S-Z, Zhu, J-F, Yan, J-X et al. Study on disinfectants for HBV using molecular biology techniques. In: AJ, Zuckermann ed. Viral hepatitis and liver disease. New York: Alan R Lisslnc, 1988; 226–7.Google Scholar
23.Chen, JHS, Koski, TA. Methods of testing virucides. In: SS, Block, ed. Disinfection, sterilization and preservation. 3rd ed.Philadelphia: Lea and Febiger, 1983; 981–97.Google Scholar