Abstract
Previous studies have suggested the presence of a tissue renin--angiotensin II system in the pancreas. These studies were based on the observation of several key components of the renin--angiotensin II system using molecular biological, biochemical and pharmacological approaches. In the present study, angiotensin II was localized immunohistochemically in the mouse pancreas using an indirect immunoperoxidase-staining technique. The results showed that angiotensin II-like immunoreactivity was localized predominantly in the endothelial cells of pancreatic blood vessels and the epithelial cells of pancreatic ducts from a subgroup of the vessels and ducts. Compared with those found in the pancreatic blood vessels and ductal system, a less pronounced immunoreactivity for angiotensin II was also observed in the acinar cells and in the smooth muscle layers overlying the pancreatic ducts as well as the blood vessels. However, no angiotensin II-like immunoreactivity was detected in the islet cells. Taken together with previous findings, the present results suggest a local angiotensin II-forming system in the mouse pancreas, which may be a significant autocrine or paracrine modulator of diverse pancreatic functions, including regulation of pancreatic blood flow and pancreatic anion secretion
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References
Barker, S., Marchant, W., Ho, M.M., Puddefoot, J.R., Hinson, J.P., Clark, A. J.L. & Vinson, G.P. (1993) A monoclonal antibody to a conserved sequence in the extracellular domain recognizes the angiotensin II AT1 receptor in mammalian target tissues. J. Mol. Endocrinol. 11, 241–5.
Boulanger, C.M., Caputo, L. & LÉ vy, B. I. (1995) Endothelial AT1-mediated release of nitric oxide decreases angiotensin II contractions in rat carotid artery. Hypertension 26, 752–7.
Chan, H.C., Law, S.H., Leung, P.S., Fu, L.X.M. & Wong, P.Y.D. (1997) Angiotensin II receptor type I-regulated anion secretion in cystic fibrosis pancreatic duct cells. J. Memb. Biol. 156, 241–9.
Chappel, M.C., Milsted, A., Diz, D. I., Brosnihan, K.B. & Ferrario, C.M. (1991) Evidence for an intrinsic angiotensin system in the canine pancreas. J. Hypertens. 9, 751–9.
Chappel, M.C., Diz, D. I. & Jacobsen, D.W. (1992) Pharmacological characterization of angiotensin II binding sites in the canine pancreas. Peptides 13, 311–18.
Coppola, S. & FrÖmter, E. (1994) An electrophysiological study of angiotensin II regulation of Na-HCO3 cotransport and K conductance in renal proximal tubules. Pflügers Arch. 427, 143–50.
Cox, H.M., Cuthbert, A.W. & Munday, K.A. (1987) The effect of angiotensin II upon electrogenic ion transport in rat intestinal epithelia. Br. J. Pharmacol. 90, 393–401.
Ghiani, B.U. & Masini, M.A. (1995) Angiotensin II binding sites in the rat pancreas and their modulation after sodium loading and depletion. Comp. Biochem. Physiol. 111A, 439–44.
Leung, P.S., Chan, H.C., Fu, L.X.M. & Wong, P.Y.D. (1997a) Localization of angiotensin II receptor subtypes AT1 and AT2 in the pancreas of rodents. J. Endocrinol. 153, 269–74.
Leung, P.S., Chan, H.C., Fu, L.X.M., Leung, P.Y., Cheng Chew, S.B. & Wong, P.Y.D. (1997b) Angiotensin II receptors: localization of type I and type II in rat epididymides of different developmental stages. J. Membr. Biol. 157, 97–103.
Leung, P.S., Chan, H.C., Fu, L.X.M., Zhou, W.L. & Wong, P.Y.D. (1997c) Angiotensin II receptors, AT1 and AT2 in the rat epididymis: immunocytochemical and electrophysiological studies. Biochim. Biophys. Acta 1357, 65–72.
Lin, L. & Nasjletti, A. (1991) Role of endotheliumderived prostanoid in angiotensin-induced vasoconstriction. Hypertension 18, 158–64.
Manabe, K., Shirahare, H., Usui, H., Kurahashi, K. & Fujiwara, M. (1989) Endothelium-dependent contractions induced by angiotensin I and angiotensin II in canine cerebral artery. J. Pharmacol. Exp. Ther. 251, 317–20.
Norris, B., Gonzalez, C., Concha, J., Palacios, S. & Contreras, G. (1991) Stimulatory effect of angiotensin II on electrolyte transport in canine tracheal epithelium. Gen. Pharmacol. 22, 527–31.
Peach, M. J. (1977) Renin-angiotensin system: biochemistry and mechanisms of action. Physiol. Rev. 57, 313–70.
Phillips, M. I., Speakman, E.A. & Kimura, B. (1993) Levels of angiotensin and molecular biology of the tissue renin-angiotensin systems. Regul. Pept. 43, 1–20.
Wong, P.Y.D. & Uchendu, C.N. (1990) The role of angiotensin-converting enzyme in the rat epididymis. J. Endocrinol. 125, 457–65.
Wong, P.Y.D. & Uchendu, C.N. (1991) Studies on the renin-angiotensin system in primary monolayer cell cultures of the rat epididymis. J. Endocrinol. 131, 287–93.
Wong, P.Y.D., Fu, W.O., Huang, S. J. & Law, W.K. (1990) Effect of angiotensins on electrogenic anion transport in monolayer cultures of rat epididymis. J. Endocrinol. 125, 449–56.
Yen, M.H., Sheu, Y.Z., Chiou, W.F. & Wu, C.C. (1990) Differential modulation by basilar and mesenteric endothelium of angiotensin-induced contraction in canine arteries. Eur. J. Pharmacol. 180, 209–16.
Zhao, W., Leung, P.Y., Cheng Chew, S.B., Chan, H.C. & Wong, P.Y.D. (1996) Localization and distribution of angiotensin II in the rat epididymis. J. Endocrinol. 149, 217–22.
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Leung, P.S., Chan, H.C. & Wong, P.Y.D. Immunohistochemical localization of angiotensin II in the mouse pancreas. Histochem J 30, 21–25 (1998). https://doi.org/10.1023/A:1003210428276
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DOI: https://doi.org/10.1023/A:1003210428276