Skip to main content
SpringerLink
Log in

Eosinophil Activation in Ulcerative Colitis (Studies on Mucosal Release and Localization of Eosinophil Granule Constituents)

  • Published:
Digestive Diseases and Sciences Aims and scope Submit manuscript

Abstract

Activation of eosinophil granulocytes(eosinophils) seems to contribute to the pathophysiologyof several inflammatory conditions. This process wasevaluated in 18 patients with ulcerative colitis and in 18 healthy controls using intraluminalsegmental perfusion of the sigmoid colon and rectum andimmunoanalysis for eosinophil cationic protein (ECP) inthe perfusate. Immunohistochemistry for eosinophils and neutrophils was made in simultaneouslytaken biopsies and in biopsies from surgical specimenstaken from additional 10 patients. The mucosal releaseof ECP was increased severalfold in patients with UC. The bowel biopsies demonstrated a laminapropria infiltrated with eosinophils. The degree ofeosinophil activation/degranulation was related to theintensity of the inflammatory reaction. Activated eosinophils and extracellular deposits of ECPwere, in particular, seen in crypt abscesses and inareas with damaged surface epithelium. Since ECP ishighly cytotoxic, its release at the site ofinflammatory bowel lesions might reflect a potentialpathophysiological mechanism.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

REFERENCES

  1. Gleich GJ, Adolpson CR: The eosinophil leucocyte: Structure and function. Adv Immunol 39:177-253, 1986

    Google Scholar 

  2. Hällgren R, Venge P: The eosinophil in inflammation. InClinical Impact of the Monitoring of Allergic Inflammation. P Venge, P Ahlstedt (eds). New York, Academic Press, 1991, pp 119-140

    Google Scholar 

  3. Wardlaw AJ: Eosinophils in the 1990s: New perspectives on their role in health and disease. Postgrad Med J 70:536-552, 1994

    Google Scholar 

  4. Gleich GJ, Loegering DA, Mann KG, Maldonado JE: Comparative properties of the Charcot-Leyden crystal protein and the major basic protein from human eosinophils. J Clin Invest 57:633- 640, 1976

    Google Scholar 

  5. Olsson I, Venge P, Spitznagel JK, Lehrer RI: Arginine-rich cationic proteins of human eosinophil granules. Comparison of the constituents of eosinophilic and neutrophilic leucocytes. Lab Invest 36:493- 500, 1977

    Google Scholar 

  6. Venge P, Dahl R, Fredens K, Hällgren R: Eosinophil cationic protein (ECP and EPX) in health and disease. InImmunology of the Eosinophil. T Yoshida, M Torisu (eds). New York, Elsevier Science Publishing, 1983, pp 163- 177

    Google Scholar 

  7. Gleich GJ, Frigas EL, Loegering DA, Wassom DL, Steinmuller D: Cytotoxic properties of the eosinophil major basic protein. J Immunol 123:2925- 2927, 1979

    Google Scholar 

  8. Tai P-C, Haye s DJ, Clark JB, Spry CJF: The e ffects of human eosinophil secretion products on isolated rat heart ce lls in vitro. Biochem J 204:75- 80, 1982

    Google Scholar 

  9. Young JD-E, Peterson CGB, Venge P, Cohn ZA: Mechanism of membrane damage mediated by human eosinophil cationic protein. Nature 321:613- 618, 1986

    Google Scholar 

  10. Makiyama K, Kanzaki S, Yamasaki K, Ze a-Iriarte W, Tsuji Y: Activation of eosinophils in the pathophysiology of ulcerative colitis. J Gastroente rol 30( suppl 8):64 - 69, 1995

    Google Scholar 

  11. Choy MY, Walker Smith JA, Williams CB, MacDonald TT: Activated eosinophils in chronic inflammatory bowel disease. Lance t 336( 8707):126 - 127, 1990 ( letter)

    Google Scholar 

  12. Berstad A, Borkje B, Riedel B, Elsayed S, Berstad A: Incre ased fecal eosinophil cationic protein in inflammatory bowel disease. Hepatogastroenterology 40:276 - 278, 1993

    Google Scholar 

  13. Garland CF, Lilienfeld AM, Mendeloff AI, Markowitz JA, Terrell KB, Garland FC: Incidence rates of ulcerative colitis and Crohn's disease in 15 areas of the United States. Gastroente rology 81:1115- 1124, 1981

    Google Scholar 

  14. Binder V: A comparison between clinical state, macroscopic and microscopic appe arance s of rectal mucosa, and cytologic picture of mucosal exudate in ulcerative colitis. Scand J Gastroenterol 5:627- 632, 1970

    Google Scholar 

  15. Raab Y, Hällgren R, Knutson L, Krog M, Gerdin B: A technique for segmental rectal and colonic perfusion in man. Am J Gastroente rol 87:1453- 1459, 1992

    Google Scholar 

  16. Tai P-C, Spry CJF, Pe terson CGB, Venge P, Olsson I: Monoclonal antibodies distinguish between storage and secrete d forms of eosinophil cationic protein. Nature 309:182- 184, 1984

    Google Scholar 

  17. Larsson L-I: Immunohistochemistry: Theory and Practice. Boca Raton, Florida, CRC Press, 1988

    Google Scholar 

  18. Hflllgren R, Colombel JF, Dahl R, Fredens K, Kruse A, Jacobsen NO, Venge P, Rambaud JC: Neutrophil and eosinophil involveme nt of the small bowel in patients with celiac disease and Crohn's disease: Studies on the secretion rate and immunohistochemical localization of granulocyte granule constituents. Am J Med 86:56 - 64, 1989

    Google Scholar 

  19. Lavö B, Knutsson L, Loööf L, Odlind B, Venge P, Hä llgren R: Challenge with gliadin induces eosinophil and mast ce ll activation in the jejunum of patients with celiac disease. Am J Med 87:655- 660, 1989

    Google Scholar 

  20. Schreiber S, MacDe rmott RP, Raedle r A, Pinnau R, Be rtovich MJ, Nash GS: Incre ased activation of isolated intestinal lamina propria mononuclear ce lls in inflammatory bowel disease. Gastroenterology 101:1020 - 1030, 1991

    Google Scholar 

  21. Torisu M, Yoishida T, Ward PA, Cohen S: Lymphocytederived eosinophil chemotactic factor. II. Studies on the me chanism of the activation of the precursor substance by immune complexe s. J Immunol 111:1450 - 1458, 1973

    Google Scholar 

  22. Ponzio NM, Speirs RS: Lymphoid ce ll dependence of eosinophil response to antigen. VI. The e ffect of re lative removal of T and B lymphocyte s on the capacity of primed spleen ce lls to adoptively transferred immunity to tetanus toxoid. Immunology 28:243- 251, 1975

    Google Scholar 

  23. Lopez AF, Sanderson CJ, Gamble JR, Campbell HD, Young IG, Vadas MA: Recombinant human interleukin 5 is a se lective activator of human eosinophil function. J Exp Med 167:219 - 224, 1988

    Google Scholar 

  24. Hällgren R, Venge P, Cullhed I, Olsson I: Blood eosinophils and eosinophil cationic protein in serum. Studies during acute myocardial infarction of corticosteroid administration. Br J Haematol 42:147- 154, 1979

    Google Scholar 

  25. Hällgren R, Fe ltelius N, Svenson K, Venge P: Eosinophil activation in rheumatoid arthritis as refle cted by elevated se rum leve ls of eosinophil cationic protein. Clin Exp Immunol 59:539 - 545, 1985

    Google Scholar 

  26. Fe ltelius N, Hällgren R, Venge P: Elevated circulating levels of eosinophil cationic protein in ankylosing spondylitis: Re lationship with the inflammatory activity and the influence of sulphasalazine treatment. Ann Rheum Dis 46:403- 407, 1987

    Google Scholar 

  27. Hubscher T: Role of the eosinophil in the allergic reactions. II. Re lease of prostaglandin from human eosinophilic leukocyte s. J Immunol 114:1389 - 1393, 1975

    Google Scholar 

  28. Ze iger RS, Twarog FJ, Colten HR: Histaminase rele ase from human granulocyte s. J Exp Med 144:1049 - 1061, 1976

    Google Scholar 

  29. Tuirk J, Maas RL, Brash AR, Roberts LJ, Oate s JA: Arachidonic acid 15-lipoxygenase products from human eosinophils. J Biol Chem 257:7068 - 7076, 1982

    Google Scholar 

  30. Welle r PF, Goetzel EJ: The human eosinophil: Role in host defense and tissue injury. Am J Pathol 100:793- 820, 1980

    Google Scholar 

  31. Beeken WL, Northwood I, Be liveau C, Baige nt G, Gump D: Eosinophils of human colonic mucosa: C3b and Fc gamma receptor expression and phagocytic capabilities. Clin Immunol Immunopathol 43:289 - 300, 1987

    Google Scholar 

  32. Beeken WL, Northwood I, Be liveau C, Gump D: Phagocyte s in ce ll suspensions of human colon mucosa. Gut 28:976 - 980, 1987

    Google Scholar 

  33. Re snick MB, Colgan SP, Patapoff TW, Mrsny RJ, Awtrey CS, Delp Archer C, Welle r PF, Madara JL: Activated eosinophils evoke chloride secretion in mode l intestinal epithelia primarily via regulated re lease of 59-AMP. J Immunol 151:5716 - 5723, 1993

    Google Scholar 

  34. Tai P-C, Holt ME, Denny P, Gibbs AR, Williams BD, Spry CJ: Deposition of eosinophil cationic protein in granulomas in allergic granulomatosis and vasculitis: The Churg-Strauss syndrome. Br Med J 289:400 - 402, 1984

    Google Scholar 

  35. Hällgren R, Bohman S-O, Fredens K: Activated eosinophil infiltration and deposits of eosinophil cationic protein in renal allograft rejection. Nephron 59:266 - 270, 1991

    Google Scholar 

Download references

Authors
  1. Yngve Raab
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kjeld Fredens
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Bengt Gerdin
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Roger Hallgren
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Raab, Y., Fredens, K., Gerdin, B. et al. Eosinophil Activation in Ulcerative Colitis (Studies on Mucosal Release and Localization of Eosinophil Granule Constituents). Dig Dis Sci 43, 1061–1070 (1998). https://doi.org/10.1023/A:1018843104511

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1018843104511

Search

Navigation