Abstract
This study was performed to define thebiologically active growth modulators in human gastricjuice. Mitogenic activity was evaluated by theincorporation of [3H]thymidine into 3T3fibroblasts. A negative correlation was observed between pH andmitogenic activity in gastric juice (r = –0.45, P< 0.01). The concentrations of epidermal growthfactor (EGF), transforming growth factor-α and-β1 (TGF-α and -β1), platelet-derived growth factor 1(PDGF), and basic fibroblast growth factor (bFGF) ingastric juice did not explain these changes in mitogenicactivity. Gel filtration identified growth-stimulatingactivity due to small molecule mitogens (less than 13kDa), and growth inhibitory activity only in neutralsamples due to a macromolecular substance (larger than240 kDa) susceptible to trypsin digestion and heat and acid treatments. We conclude thatacidity-dependent changes in mitogenic activity observedin this study are due to appearance of acid-unstable,high-molecular-weight, growth-inhibitorysubstance.
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Kataoka, H., Joh, T., Kasugai, K. et al. Mitogenic Properties of Human Gastric Juice. Dig Dis Sci 42, 1747–1754 (1997). https://doi.org/10.1023/A:1018877918894
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DOI: https://doi.org/10.1023/A:1018877918894