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Effect of diet and exercise on serum insulin, IGF-I, and IGFBP-1 levels and growth of LNCaP cells in vitro (United States)

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Abstract

Objective: Accumulating evidence indicates that prostate cancer is associated with high levels of serum IGF-I. This study was conducted to determine whether a low-fat diet and exercise (DE) intervention may modulate the IGF axis and reduce prostate cancer cell growth in vitro. Methods: Fasting serum was obtained from 14 men (age 60 ± 3 years) participating in an 11-day DE program and from eight similarly aged men who had followed the DE program for 14.2 ± 1.7 years (long-term). Insulin, IGF-I, IGFBP-1, and IGFBP-3 were measured by ELISA, and serum was used to stimulate LNCaP cell growth in vitro. Results: Serum IGF-I levels decreased by 20% while IGFBP-1 increased by 53% after 11-day DE. In the long-term group, IGF-I was 55% lower, while IGFBP-1 was 150% higher relative to baseline. Serum insulin decreased by 25% after 11-day DE and was 68% lower in the long-term group, relative to baseline. No changes in serum IGFBP-3 were observed. Serum-stimulated LNCaP cell growth was reduced by 30% in post-11-day serum and by 44% in long-term serum relative to baseline. LNCaP cells incubated with post-DE serum showed increased apoptosis/necrosis, compared to baseline. Conclusions: A low-fat diet and exercise intervention induces in-vivo changes in the circulating IGF axis and is associated with reduced growth and enhanced apoptosis/necrosis of LNCaP tumor cells in vitro.

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Authors and Affiliations

  1. Departments of Physiological Science, University of California, Los Angeles, CA, USA

    Tung H. Ngo, R. James Barnard & Christopher N. Tymchuk

  2. Department of Physiological Science, University of California, Los Angeles, 3219

    R. James Barnard

  3. Life Science Building, 621 Charles Young Dr. So, Los Angeles, CA, 90095, USA

    R. James Barnard

  4. Pediatrics, University of California, Los Angeles, CA, USA

    Pinchas Cohen

  5. Urology, University of California, Los Angeles, CA, USA

    William J. Aronson

Authors
  1. Tung H. Ngo
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  2. R. James Barnard
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  3. Christopher N. Tymchuk
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  4. Pinchas Cohen
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  5. William J. Aronson
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Ngo, T.H., Barnard, R.J., Tymchuk, C.N. et al. Effect of diet and exercise on serum insulin, IGF-I, and IGFBP-1 levels and growth of LNCaP cells in vitro (United States). Cancer Causes Control 13, 929–935 (2002). https://doi.org/10.1023/A:1021911517010

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