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Role of NF-κB and AP-1 on Helicobater pylori-Induced IL-8 Expression in AGS Cells

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Abstract

Oxygen radicals are important regulators in Helicobacter pylori-induced gastric ulceration and carcinogenesis. IL-8 may be regulated by oxidant-sensitive transcription factors, NF-κB, and AP-1. The present study aims to investigate whether H. pylori-induced IL-8 expression is regulated by NF-κB and AP-1 in gastric epithelial AGS cells and whether this transcriptional regulation of IL-8 is inhibited by N-acetylcysteine (NAC). As a result, H. pylori induced the expression of mRNA and protein for IL-8 via activation of NF-κB and AP-1. NF-κB activation accompanied by a decrease in I-κBα and activated AP-1 complex was a c-jun/c-fos heterodimer in H. pylori-infected AGS cells. NAC inhibited H. pylori-induced activation of transcription factors and IL-8 expression in AGS cells. In conclusion, oxygen radicals induce the activation of NF-κB and AP-1 and IL-8 expression. Antioxidants such as NAC might be useful anti-inflammatory agents by inhibiting activation of transcription factors and decreasing IL-8 production in H. pylori-induced gastric inflammation.

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Authors and Affiliations

  1. Department of Pharmacology and Institute of Gastroenterology, Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, Seoul, Korea

    Sang Hui Chu, Hyeyoung Kim, Jeong Yeon Seo, Joo Weon Lim & Kyung Hwan Kim

  2. Department of Molecular Oncology, Cancer Research Institute, Kanazawa University, Kanazawa, Japan

    Naofumi Mukaida

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  1. Sang Hui Chu
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  2. Hyeyoung Kim
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  5. Naofumi Mukaida
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Chu, S.H., Kim, H., Seo, J.Y. et al. Role of NF-κB and AP-1 on Helicobater pylori-Induced IL-8 Expression in AGS Cells. Dig Dis Sci 48, 257–265 (2003). https://doi.org/10.1023/A:1021963007225

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