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Cloning and expression of human tissue-type plasminogen activator cDNA in E. coli

Nature volume 301, pages 214–221 (1983)Cite this article

Abstract

Bacterial clones containing human tissue-type plasminogen activator (t-PA) cDNA sequences were identified in a cDNA library prepared using gel-fractionated mRNA from human melanoma cells. A plasmid containing the Escherichia coli trp promoter and the cDNA sequence coding f or the 527-amino acid mature t-PA protein was constructed for expression in E. coli. A polypeptide was produced having the fibrinolytic properties characteristic of authentic human t-PA.

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Author information

Authors and Affiliations

  1. Department of Molecular Biology, Genentech, Inc., 460 Point San Bruno Boulevard, South San Francisco, California, 94080, USA

    Diane Pennica, William E. Holmes, Elizabeth Yelverton, Peter H. Seeburg, Herbert L. Heyneker & David V. Goeddel

  2. Department of Protein Biochemistry, Genentech, Inc., 460 Point San Bruno Boulevard, South San Francisco, California, 94080, USA

    William J. Kohr, Richard N. Harkins, Gordon A. Vehar, Carole A. Ward & William F. Bennett

  3. Center for Thrombosis and Vascular Research, Department of Medical Research, University of Leuven, B-3000, Leuven, Belgium

    Desire Collen

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  1. Diane Pennica
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Pennica, D., Holmes, W., Kohr, W. et al. Cloning and expression of human tissue-type plasminogen activator cDNA in E. coli. Nature 301, 214–221 (1983). https://doi.org/10.1038/301214a0

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