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Lipopolysaccharide increases cyclo-oxygenase-2 expression in a colon carcinoma cell line through nuclear factor-κB activation

Oncogene volume 19, pages 1225–1231 (2000)Cite this article

Abstract

Both nonneoplastic colon epithelium and colon carcinoma cells in situ are continuously exposed to lipopolysaccharide (LPS). Few reports have addressed possible direct effects of LPS in promotion of colon carcinoma and underlying mechanisms. We found evidence that LPS directly stimulated growth of the human colon carcinoma cell line CE-1 through an increase in the production of prostaglandin E2 (PGE2) as a result of cyclo-oxygenase-2 (COX-2) expression. LPS induced significant increases in PGE2 production in CE-1 cells, which were found to express a high-affinity LPS receptor, CD14. Positive correlations were found between PGE2 production and activation of nuclear factor (NF)-κB as well as expression of both COX-2 mRNA and protein in LPS-stimulated CE-1 cells. When CE-1 cells were exposed to exogenous PGE2, DNA synthesis increased. These results indicate that LPS may stimulate DNA synthesis in certain colon carcinoma cells as a result of PGE2 production involving increased COX-2 expression that might result in turn from activation of NF-κB by LPS. Further investigation of the pathways mediating LPS-induced stimulation of colon carcinoma cells may provide insights into LPS effects in in vivo tumor biology.

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Abbreviations

LPS:

lipopolysaccharide

PG:

prostaglandin

COX:

cyclo-oxygenase

NF-κB:

nuclear factor-κB

RT–PCR:

reverse transcription-polymerase chain reaction

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Acknowledgements

We thank Ms Sumiko Sakamoto for technical assistance.

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Authors and Affiliations

  1. Division of Clinical Immunology, First Department of Surgery, Faculty of Medicine, Kyushu University, 3-1-1 Maidashi, Higashi-ku, 812-8582, Fukuoka, Japan

    Masayuki Kojima, Akihiko Uchiyama, Yasuo Matsunari & Masao Tanaka

  2. Department of Clinical Chemistry and Laboratory Medicine, Faculty of Medicine, Kyushu University, 3-1-1 Maidashi, Higashi-ku, 812-8582, Fukuoka, Japan

    Kenji Izuhara

  3. Department of Cancer Therapy and Research, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, 812-8582, Fukuoka, Japan

    Takashi Morisaki & Mitsuo Katano

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  1. Masayuki Kojima
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Kojima, M., Morisaki, T., Izuhara, K. et al. Lipopolysaccharide increases cyclo-oxygenase-2 expression in a colon carcinoma cell line through nuclear factor-κB activation. Oncogene 19, 1225–1231 (2000). https://doi.org/10.1038/sj.onc.1203427

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