Hyperthermia induces heat shock protein expression and protection against cerulein-induced pancreatitis in rats
Abstract
BACKGROUND & AIMS: Heat shock proteins (HSPs) are part of the cellular stress response machinery. HSPs are expressed in the pancreas, but their protective potential has not been thoroughly investigated. The aim of this study was to characterize the pancreatic heat shock response both in vitro and in vivo and to study whether this response has protective effects. METHODS: For in vitro experiments, rat pancreatic acini were exposed to heat and protein expression was analyzed through 35S-methionine labeling or Western blots. In vivo, cerulein pancreatitis was induced after whole body hyperthermia (42 degrees C). RESULTS: After thermal stress (42 degrees C), acini increasingly expressed five proteins of 92, 72, 59, 58, and 30 kilodaltons, with HSP. 70 the most strongly induced 72-kilodalton protein. In vivo, increased expression of four isoforms of pancreatic HSP-70 was found. Isoform-specific antibodies identified the inducible and constitutively expressed (HSC-70) isoform of HSP-70. HSP-60 was also weakly induced after hyperthermia. Concomitantly, cerulein-induced pancreatic organ damage was greatly reduced after whole-body hyperthermia. The time course and strength of HSP induction correlated well with the time course and degree of protection against cerulein- induced pancreatitis. CONCLUSIONS: A causal relation between hyperthermia-induced HSP expression and organ protection seems possible. (Gastroenterology 1996 Nov;111(5):1333-42)
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Recent advances in studies of molecular hydrogen in the treatment of pancreatitis
2021, Life SciencesCitation Excerpt :In mouse macrophages research administrated with LPS, Hsc70 could attenuate NO production, mitigate TNF-α and IL-6 expression, diminish NF-κB nuclear translocation, and reduce the phosphorylation of ERK1/2, JNK, p38 MAPK, and phosphodylinositol-3-kinase (PI3K) [78]. It has been proved that the upregulation of Hsc70 can protect against pancreatitis [79]. The study found that Hsc70 in AR42J cells induced by caerulein was increased, and H2 further upregulated its expression [8].
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Alteration of Cpn60 expression in pancreatic tissue of rats with acute pancreatitis
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Dual, time-dependent deleterious and protective effect of anandamide on the course of cerulein-induced acute pancreatitis. Role of sensory nerves
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