Clinical–Liver, Pancreas, and Biliary TractAberrant expression of MUC5AC and MUC6 gastric mucins and sialyl Tn antigen in intraepithelial neoplasms of the pancreas☆,☆☆
Section snippets
Specimen selection and identification of pancreatic intraepithelial neoplasias
Representative paraffin-embedded tissue blocks from 20 selected cases of infiltrating adenocarcinoma were obtained from the Department of Pathology at the University of Chicago and from 48 cases from the Department of Anatomic Pathology at the University of California, San Francisco. H&E-stained sections from each case were screened by light microscopy for PanINs and infiltrating carcinomas. PanIN lesions were identified and graded by 2 pathologists. Criteria established at a National Cancer
Normal pancreas
By using immunohistochemical methods, only weak focal expression of all mucin proteins was observed in the intercalating ducts. MUC1 protein was expressed in 18% and 23% of normal intralobular and interlobular ducts, respectively, whereas MUC6 protein was expressed in 12% and 27% of normal intralobular and interlobular ducts, respectively. MUC2 was not expressed in normal ducts. Both MUC5AC protein and Nd2-defined antigen were expressed at very low frequency, 4% and 2%, respectively. MUC1
Discussion
Recent advances in physicochemical, immunochemical, and molecular biologic methods have increased our understanding of the structures of both carbohydrate and peptide moieties of mucin glycoproteins.1, 2, 9 To date, at least 14 mucin genes have been identified and characterized and these mucin glycoproteins are broadly classified into secreted or membrane-associated forms. In normal tissues, mucin genes are expressed in a cell- and tissue-type–specific fashion.1, 2, 4, 5, 6, 7, 41, 43, 44 For
Acknowledgements
The authors thank David Geller for his editorial assistance.
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Address requests for reprints to: Young S. Kim, M.D., VA Medical Center, GI Research Laboratory (151M2), 4150 Clement Street, San Francisco, California 94121. e-mail: [email protected]; fax: (415) 750-6972.
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Supported by United States Public Health Service grant CA24321 from the National Cancer Institute, an Oberkotter Foundation grant, and by the Veterans Affairs Medical Research Service.