Basic-alimentary tractNerve growth factor mediates alterations of colonic sensitivity and mucosal barrier induced by neonatal stress in rats☆
Section snippets
Animals
Primiparous pregnant female Wistar rats were individually housed in standard polypropylene cages containing 2.5 cm of wood chip bedding material. Rats were kept in a constant-temperature room (23°C ± 1°C) and maintained on a 12-hour light/dark cycle (lights on at 7 am). Food (UAR pellets; Epinay, France) and water were available ad libitum. Mothers and their pups, as well as the young rats after weaning on day 22, were kept in the same conditions.
MD
MD was performed according to a previously
Visceral sensitivity to RD
Gradual RD increased the frequency of abdominal contractions in a distention volume-dependent manner. In nondeprived rats, a volume of 0.8 mL was the threshold at which RD significantly increased (P < 0.05) the number of abdominal contractions compared with the predistention period. In deprived rats that received no injection, this threshold was significantly reduced (P < 0.05) to 0.4 mL (Figure 1).
Compared with nondeprived controls, neonatal MD significantly increased (P < 0.05) the number of
Discussion
This study provides new evidence that NGF plays a major role in triggering (neonatal period) and maintaining long-term visceral hyperalgesia and alteration of the colonic epithelial barrier in neonatally stressed rats by MD. We also show that mast cell degranulation participates in the mucosal and sensitivity alterations induced by neonatal MD. The first part related to the role of NGF in neonatal stress is supported by our data showing that the effects of neonatal separation are suppressed by
Acknowledgements
The authors thank Bernard Joseph for technical assistance.
References (59)
- et al.
Psychosocial aspects of Crohn’s disease
Surg Clin North Am
(2001) - et al.
Predisposition to gastric erosions in the ratbehavioral and nutritional effects of early maternal separation
Gastroenterology
(1978) - et al.
Tolerance for rectosigmoid distention in irritable bowel syndrome
Gastroenterology
(1990) - et al.
Early maternal separation increases NGF expression in the developing rat hippocampus
Pharmacol Biochem Behav
(1998) - et al.
NGF expression in the developing rat braineffects of maternal separation
Brain Res Dev
(2000) Role of environmental factors on brain development and nerve growth factor expression
Physiol Behav
(2001)- et al.
Hyperalgesia due to nerve damagerole of nerve growth factor
Pain
(1999) - et al.
Mast cells and gut nerve developmentimplications for Hirschsprung’s disease and intestinal neuronal dysplasia
J Pediatr Surg
(1999) - et al.
Neurotrophinssignals between the nervous and immune systems
Prog Brain Res
(1994) - et al.
p75 and Trka two-receptor system
Trends Neurosci
(1995)
Electrical spiking activity and propulsion in small intestine in fed and fasted rats
Gastroenterology
Measurement of cutaneous inflammationestimation of neutrophil content with an enzyme marker
J Invest Dermatol
Chronic low-level administration of diquat increases the nociceptive response to gastric distension in ratsrole of mast cells and tachykinin receptor activation
Pain
Nerve growth factor enhances neurite arborization of adult sensory neurons; a study in single-cell culture
Brain Res
Nerve growth factor (NGF) regulates tachykinin gene expression and biosynthesis in rat sensory neurons during early postnatal development
Neuropeptides
Differential effects of treatment with nerve growth factor on thermal nociception and on calcitonin gene-related peptide content of primary afferent neurons in the rat
Neurosci Lett
Sensitisation of visceral afferents by nerve growth factor in the adult rat
Pain
Role of nerve growth factor in the trinitrobenzene sulfonic acid-induced colonic hypersensitivity
Pain
Attenuation of nerve growth factor-induced visceral hyperalgesia via cannabinoid CB(1) and CB(2)-like receptors
Pain
Nerve growth factor depletion by autoimmunization produces thermal hypoalgesia in adult rats
Brain Res
Rapid induction of neurotrophin mRNAs in rat glial cell cultures by Semax, an adrenocorticotropic hormone analog
Neurosci Lett
Proteinases and proteinase-activated receptor 2a possible role to promote visceral hyperalgesia in rats
Gastroenterology
Nerve growth factor induces the expression of certain cytokine genes and bcl-2 in mast cells. Potential role in survival promotion
J Biol Chem
Childhood deprivationan antecedent of the irritable bowel syndrome
Med J Aust
Recollection of childhood events in adults with irritable bowel syndrome
J Clin Gastroenterol
Neonatal maternal separation predisposes adult rats to colonic barrier dysfunction in response to mild stress
Am J Physiol
Neonatal maternal separation alters stress-induced responses to viscerosomatic nociceptive stimuli in rat
Am J Physiol
Influence of sex and experimental protocol on the effect of maternal deprivation on rectal sensitivity to distension in the adult rat
Neurogastroenterol Motil
Physiology of the neurotrophins
Annu Rev Neurosci
Cited by (0)
- ☆
Supported by the Institut National de la Recherche Agronomique (Paris, France).