Basic—Alimentary TractSleeve Gastrectomy Induces Loss of Weight and Fat Mass in Obese Rats, but Does Not Affect Leptin Sensitivity
Section snippets
Animals
Male and female Long–Evans rats (Harlan Laboratories, Indianapolis, IN; 250–300 g) were fed either a high-fat butter oil-based diet (HFD; Research Diets, New Brunswick, NJ, D12451; 45% fat; 4.73 kcal/g) or standard chow (Harlan-Teklad, Indianapolis, IN) for 8 weeks prior to surgery. Rats were housed at the University of Cincinnati at the Metabolic Diseases Institute under controlled conditions (12:12-hour light–dark cycle, 50%–60% humidity, 25°C with free access to water and food except where
Weight Loss and Fat Mass Loss Following VSG Persist Despite Transient Reductions in Food Intake
Following surgery, VSG rats lost about 20% of initial weight (Figure 1A and B). After the initial period of weight loss (10 days), VSG animals gained an average of 1.03 ± 0.11 g/day for males and 1.63 ± 0.14 g/day for females. This is comparable to weight gain in both naïve (females: 1.12 ± 0.05 g/day, males: 1.54 ± 0.14 g/day) and sham animals (females: 1.43 ± 0.10 g/day, males: 1.57 ± 0.11 g/day). Body weight remained reduced in VSG rats compared to sham and naïve rats throughout the duration
Discussion
The current rat model of VSG produces significant and durable weight loss in obese male and female rats. Importantly, this rodent model of VSG leads the rat to defend a new, lower body weight. Unlike what is seen following weight loss by caloric restriction, rats following VSG do not overeat to compensate for this reduced fat mass. Additionally, we provide strong evidence that the weight loss cannot be attributed to an improvement in leptin sensitivity.
Weight loss secondary to VSG was due to
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Conflicts of interest These authors disclose the following:
Drs Seeley, Tschöp, Woods, and Sandoval receive funding from Johnson & Johnson.
The remaining authors disclose no conflicts.