Dendritic Cells Shuttle Microbes Across Gut Epithelial Monolayers

doi:10.1078/0171-2985-00094

Immunobiology

Volume 204, Issue 5, 2001, Pages 572-581

https://doi.org/10.1078/0171-2985-00094 Get rights and content

Summary

Understanding the mechanisms governing the type of induced immune response after microbial invasion, could be of crucial importance for the rational design of a bacteria-based vaccine. Targeting a vaccine directly to dendritic cells (DCs), which are considered the most powerful antigen presenting cells, could be extremely effective. Here we describe that CD11b⁺CD8a⁻ dendritic cells are involved in the direct bacterial uptake across mucosal surfaces. DCs are widely spread in the lamina propria of the gut and are recruited at the site of infection. DCs open the tight junctions between epithelial cells, send dendrites outside of the epithelium and sample bacteria. Moreover, the integrity of the epithelial barrier is preserved because DCs express tight junction proteins, such as occludin, claudin 1 and Junctional Adhesion Molecule (JAM) and can establish tight junctions-like structures with neighbouring epithelial cells.

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Dendritic Cells Shuttle Microbes Across Gut Epithelial Monolayers

Summary

Immunol. Today

Curr. Opin. Cell. Biol.

J. Biol. Chem.

Junctional complexes in various epithelia

Journal of Cell Biology

Structure and function of the intestinal epithelial barrier in health and disease

Monographs in Pathology

Specific adherence of Escherichia membranous (M) cells of the Peyer's patch in Escherichia coli diarrhea in the rabbit

Journal of coli (strain RDEC-1) to Clinical Investigation

Role of M cells in initial antigen uptake and in ulcer formation in the rabbit intestinal loop model of shigellosis

Infection & Immunity

Cytopathogenic effect of Salmonella typhi GIFU 10007 on M cells of murine ileal Peyer's patches in ligated ileal loops: an ultrastructural study

Microbiology & Immunology

Cloning and molecular characterization of genes whose products allow Salmonella typhimurium to penetrate tissue culture cells

Proceedings of the National Academy of Sciences of the United States of America

Dendritic cells and the control of immunity

Nature

A discrete subpopulation of dendritic cells transports apoptotic intestinal epithelial cells to T cell areas of mesenteric lymph nodes [see comments]

J. Exp. Med.

Maturation of Peyer's patch dendritic cells in vitro upon stimulation via cytokines or CD40 triggering

European Journal of Immunology

Distinct populations of dendritic cells are present in the subepithelial dome and T cell regions of the murine Peyer's patch

Journal of Experimental Medicine

Localization of distinct Peyer's patch dendritic cell subsets and their recruitment by chemokines macrophage inflammatory protein (MIP)-3alpha, MIP-3beta, and secondary lymphoid organ chemokine

J. Exp. Med.