Skip to main content

Manipulation of Adenovirus Vectors

  • Protocol
Book cover Gene Transfer and Expression Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 7))

Abstract

Adenoviruses have been isolated from a large number of different species (mammalian and fowl) and over 100 different serotypes have been reported, some 43 of them human. The human adenoviruses, particularly types 2, 5, and 12, have been the most extensively characterized, and these viruses have served as valuable tools in the study of the molecular biology of DNA replication, transcription, RNA processing, and protein synthesis in mammalian cells. Seeref. 1 for a general review.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Ginsberg, H. S. (1984) The Adenoviruses (Plenum, New York).

    Google Scholar 

  2. Berkner, K. L. (1988) Development of adenovirus vectors for expression of heterolo gous genes. BioTechnigues 6, 616–629.

    Article  CAS  Google Scholar 

  3. Jones, N. and Shenk, T. (1979) Isolation of adenovirus type 5 host range deletion mutants defective for transformation of rat embryo cells. Cell 16, 683–689.

    Article  Google Scholar 

  4. Graham, F. L., Smiley, J., Russell, W. C., and Nairn, R. (1977) Characteristics of a human cell line transformed by DNA from human adenovirus type 5. J Gen. Virol. 36, 59–72.

    Article  PubMed  CAS  Google Scholar 

  5. Ghosh-Choudhury, G., Haj-Ahmad, Y., and Graham, F. L. (1987) Protein IX, a minor component of the human adenovirus capsid, is essential for the packaging of full length genomes. EMBOJ 6, 1733–1739.

    CAS  Google Scholar 

  6. Berkner, K L. and Sharp, P. A. (1983) Generation of adenovirus by transfection of plasmids. Nucleic Acids Res. 11, 6003–6020.

    Article  PubMed  CAS  Google Scholar 

  7. Haj-Ahmad, Y. and Graham, F. L. (1986) Development of a helper independent human adenovirus vector and its use in the transfer of the Herpes Simplex Virus thy-midine kinase gene. J Krol. 57, 267–274.

    CAS  Google Scholar 

  8. Johnson, D. C., GhoshChoudhury, G., SmiIey, J. R., Fallis, L., and Graham, F. L. (1988) Abundant expression of Herpes Simplex Virus glycoprotein gB using an adenovirus vector. Virology 164, 1–14.

    Article  PubMed  CAS  Google Scholar 

  9. Schneider, M., Graham, F. L., and Prevec, L. (1989) Expression of the gIycoprotein of VSV by infectious adenovirus vectors. J. Gen, Virol. 70, 417–427.

    Article  CAS  Google Scholar 

  10. Davis, A. R., Kostek, B., Mason, B. B., Hsiao, C. L., Morin, J., Dheer, S. EC, and Hung, P. P. (1985) Expression of hepatitis B virus surface antigen with a recombinant adenovirus. Proc. Natl. Acad. Sci. USA 82, 7560–7564.

    Article  PubMed  CAS  Google Scholar 

  11. Spessot, R., Inchley, K, Hupel, T. M., and Bacchetti, S. (1989) Cloning of the herpes simplex virus ICP4 gene in an adenovirus vector: Effects on adenovirus gene expression and replication. Virolology 168, 378–387.

    Article  CAS  Google Scholar 

  12. Ruben, M., Bacchetti, S., and Graham, F. L. (1983) Covalently closed circles of human adenovirus type 5 DNA. Nature 3O1, 172–174.

    Article  Google Scholar 

  13. Graham, F. L. (1984) Covalently closed circles of human adenovirus DNA are infectious. EMBOJ 3 2917–2922.

    CAS  Google Scholar 

  14. Ghosh-Choudhury, G., Haj-Ahmad, Y., Brinkley, P., Rudy, J., and Graham, F. L. (1986)Human adenovirus cloning vectors based on infectious bacterial plasmids. Gene 50, 161–171.

    Article  PubMed  CAS  Google Scholar 

  15. McGroty, J., Bautista, D., and Graham, F. L. (1988) A simple technique for the rescue of early region I mutations into infectious human adenovirus type 5. Virology 168, 614–617.

    Article  Google Scholar 

  16. Graham, F. L. (1981) Growth of 293 cells in suspension culture. J Gen. Kirol. 68, 937–940.

    Article  Google Scholar 

  17. Sharp, P. A., Moore, C., and Haverty, J. L. (1976) The infectivity of adenovirus 5 DNA-protein complex. Virology 75, 442–456.

    Article  PubMed  CAS  Google Scholar 

  18. Chinnadurai, G., Chinnadurai, S., and Green, M. (1978) Enhanced infectivity of adenovirus type 2 DNA and a DNA-protein complex. J. Virol 26, 195–199.

    PubMed  CAS  Google Scholar 

  19. Graham, F. L. and van der Eb, A.J. (1973) A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology 52, 456–467.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1991 The Humana Press Inc., Clifton, NJ

About this protocol

Cite this protocol

Graham, F.L., Prevec, L. (1991). Manipulation of Adenovirus Vectors. In: Murray, E.J. (eds) Gene Transfer and Expression Protocols. Methods in Molecular Biology, vol 7. Humana Press. https://doi.org/10.1385/0-89603-178-0:109

Download citation

  • DOI: https://doi.org/10.1385/0-89603-178-0:109

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-178-4

  • Online ISBN: 978-1-59259-494-8

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics