Oral Medicine & Pathology
Online ISSN : 1882-1537
Print ISSN : 1342-0984
ISSN-L : 1342-0984
Original articles
HSP 47 and Angiogenic Factor Expression and Its Implication for the Healing of Periosteal Defects in the Mouse Cranium
Katsumi HideshimaJoji SekineShogo MimuraTsugio Inokuchi
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JOURNAL FREE ACCESS

2006 Volume 11 Issue 1 Pages 27-33

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Abstract

We investigated collagen formation during healing in an experimentally-created periosteal defect site (PDS) in terms of the immunohistochemical detection of Heat Shock Protein 47 (HSP47) and α -Smooth Muscle Actin ( α -SMA) along with the histopathological observation. In addition, we investigated expression of angiogenic factors, Vascular Endothelial Growth Factor (VEGF) and von Willebrand Factor (vWF), from the viewpoint of nutritional compensation for the bone in the PDS.
The histological findings showed that the PDS was filled with fibrin-like structure until 3 days postoperatively, with granulation tissue at 5 days postoperatively, and it was finally replaced by collagenous fibers. The reparative periosteum-like tissue at 84 days postoperatively, showed features similar to those of normal periosteum.
HSP47 and α -SMA showed a positive reaction in fibroblast-like cells as well as immature collagen fibers in the healing process of the PDS. At 84 days after operation, some of the fibroblast-like cells in the reparative periosteum-like tissue showed a positive reaction to HSP47 and α -SMA, similar to normal periosteum.
Some collagen fibers in the normal periosteum showed a positive reaction for vWF. Fibrin-like structure, granulation tissue including fibrobast-like cells and some collagen fibers in the PDS were positive for VEGF and vWF during the experimental term.
In conclusion, it is suggested that periosteal defects heal with regeneration of the collagenous structure, which has a role of nourishing the corresponding bone tissue.

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© 2006 The Japanese Society of Oral Pathology
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