Regulation by chemokines of circulating dendritic cell precursors, and the formation of portal tract-associated lymphoid tissue, in a granulomatous liver disease

H Yoneyama; K Matsuno; Y Zhang; M Murai; M Itakura; S Ishikawa; G Hasegawa; M Naito; H Asakura; K Matsushima

doi:10.1084/jem.193.1.35

Regulation by chemokines of circulating dendritic cell precursors, and the formation of portal tract-associated lymphoid tissue, in a granulomatous liver disease

J Exp Med. 2001 Jan 1;193(1):35-49. doi: 10.1084/jem.193.1.35.

Authors

H Yoneyama¹, K Matsuno, Y Zhang, M Murai, M Itakura, S Ishikawa, G Hasegawa, M Naito, H Asakura, K Matsushima

Affiliation

¹ Department of Molecular Preventive Medicine, School of Medicine The University of Tokyo, Bunkyoku, Tokyo 113-0033, Japan.

Abstract

We have studied the recruitment and roles of distinct dendritic cell (DC) precursors from the circulation into Propionibacterium acnes-induced granulomas in mouse liver. During infection, F4/80(-)B220(-)CD11c(+) DC precursors appeared in the circulation, migrated into the perisinusoidal space, and matured within newly formed granulomas. Recruited DCs later migrated to the portal area to interact with T cells in what we term "portal tract-associated lymphoid tissue" (PALT). Macrophage inflammatory protein 1alpha attracted blood DC precursors to the sinusoidal granuloma, whereas secondary lymphoid organ chemokine (SLC) attracted mature DCs to the newly identified PALT. Anti-SLC antibody diminished PALT expansion while exacerbating granuloma formation. Therefore, circulating DC precursors can migrate into a solid organ like liver, and participate in the granulomatous reaction in response to specific chemokines.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Base Sequence
CD11 Antigens / metabolism
Cell Movement / drug effects
Cell Movement / immunology
Chemokine CCL21
Chemokine CCL4
Chemokines / genetics
Chemokines / pharmacology*
Chemokines / physiology
Chemokines, CC / genetics
Chemokines, CC / physiology
DNA Primers / genetics
Dendritic Cells / drug effects
Dendritic Cells / immunology*
Dendritic Cells / pathology*
Female
Granuloma / immunology*
Granuloma / pathology*
Liver Diseases / immunology*
Liver Diseases / pathology*
Lymphoid Tissue / drug effects
Lymphoid Tissue / immunology
Lymphoid Tissue / pathology
Macrophage Inflammatory Proteins / genetics
Macrophage Inflammatory Proteins / physiology
Mice
Mice, Inbred BALB C
Mice, Inbred C3H
Mice, Inbred C57BL
Mice, Mutant Strains
Propionibacterium acnes / pathogenicity
Stem Cells / drug effects
Stem Cells / immunology
Stem Cells / pathology

Substances

CD11 Antigens
Ccl21c protein, mouse
Chemokine CCL21
Chemokine CCL4
Chemokines
Chemokines, CC
DNA Primers
Macrophage Inflammatory Proteins