Abstract
Because the prognosis of patients with pancreatic cancer is very poor, development of a novel approach for treatment of this disease is vital. In the present study, we investigated the effect of dendritic cell (DC)-based immunotherapy against established syngeneic hamster pancreatic cancer named HPD1NR. Hamster enriched DCs were prepared from bone marrow (BM) by a culture for 7 days in the presence of mouse GM-CSF and mouse IL-4, and characterized by the expression of specific DC markers (DEC205, DC-SIGN) mRNA using in situ hybridization (ISH). DCs pulsed with tumor lysate and N-[1-(2,3-dioleoyloxy)propyl]-N,N,N-trimethylammonium methylsulfate (DOTAP) or DCs alone were injected s.c. weekly into HPD1NR-bearing hamsters three times. Tumor growth was significantly inhibited by 82% in hamsters treated with tumor lysate and DOTAP-pulsed DCs when compared with the PBS vehicle-treated group. These findings suggest that DC-based immunotherapy may be a useful approach for the treatment of pancreatic cancers.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Bone Marrow / immunology
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Bone Marrow / metabolism
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Bone Marrow / pathology
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Cancer Vaccines / immunology*
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Cricetinae
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Cricetulus
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DNA Primers / chemistry
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Dendritic Cells / immunology*
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Fatty Acids, Monounsaturated
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Female
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Fluorescent Dyes
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Genetic Vectors
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Granulocyte-Macrophage Colony-Stimulating Factor / pharmacology
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Immunotherapy*
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In Situ Hybridization
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Injections, Subcutaneous
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Interleukin-12 / pharmacology
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Interleukin-4 / pharmacology
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Leukocytes / metabolism
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Mesocricetus
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Mice
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Mice, Inbred C57BL
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Neoplasm Transplantation
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Pancreatic Neoplasms / immunology
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Pancreatic Neoplasms / therapy*
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Polymerase Chain Reaction
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Quaternary Ammonium Compounds
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RNA, Messenger / metabolism
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Recombinant Proteins / pharmacology
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Spleen / metabolism
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T-Lymphocytes / immunology
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T-Lymphocytes, Cytotoxic / immunology
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Tumor Cells, Cultured
Substances
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Cancer Vaccines
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DNA Primers
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Fatty Acids, Monounsaturated
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Fluorescent Dyes
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Quaternary Ammonium Compounds
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RNA, Messenger
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Recombinant Proteins
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Interleukin-12
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Interleukin-4
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Granulocyte-Macrophage Colony-Stimulating Factor
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1,2-dioleoyloxy-3-(trimethylammonium)propane