Abstract
Using cDNA-Representational Difference Analysis it was found that expression of Opg, Ctse, Krt2-4, Fut-2, 24p3 and Wif-1 genes was elevated in intestinal adenomas as compared to normal epithelial cells of Apc(Min/+) mutant mice. Expression of Wif-1, which encodes Wnt inhibitory factor-1 was also detected in a number of tumor cell lines of epithelial cell origin including two human colon adenocarcinoma cell lines. The possible role of Wif-1 over-expression in the etiology of colorectal cancer is discussed.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adaptor Proteins, Signal Transducing
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Adenocarcinoma / metabolism
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Adenocarcinoma / pathology
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Adenoma / metabolism
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Adenoma / pathology
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Adenomatous Polyposis Coli Protein / genetics
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Adenomatous Polyposis Coli Protein / physiology*
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Animals
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Biomarkers, Tumor / genetics
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Biomarkers, Tumor / metabolism
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Carrier Proteins / metabolism*
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Cell Transformation, Neoplastic*
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Colorectal Neoplasms / metabolism
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Colorectal Neoplasms / pathology
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DNA, Complementary / genetics
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Epithelial Cells / metabolism
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Epithelial Cells / pathology*
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Extracellular Matrix Proteins
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Gene Expression Regulation, Neoplastic
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Humans
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Intercellular Signaling Peptides and Proteins
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Intestinal Mucosa / metabolism
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Intestinal Neoplasms / metabolism
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Intestinal Neoplasms / pathology*
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Intestines / pathology*
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Intracellular Signaling Peptides and Proteins
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Mice
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Mice, Inbred C57BL
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Mice, Knockout
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Neoplasm Proteins / genetics
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Neoplasm Proteins / metabolism
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Repressor Proteins / metabolism*
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Reverse Transcriptase Polymerase Chain Reaction
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Tumor Cells, Cultured
Substances
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Adaptor Proteins, Signal Transducing
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Adenomatous Polyposis Coli Protein
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Biomarkers, Tumor
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Carrier Proteins
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DNA, Complementary
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Extracellular Matrix Proteins
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Intercellular Signaling Peptides and Proteins
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Intracellular Signaling Peptides and Proteins
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Neoplasm Proteins
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RNA, Messenger
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Repressor Proteins
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WIF1 protein, human
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Wif1 protein, mouse