Abstract
The human CD4+CD25+FoxP3+ regulatory T cell population (Tregs) contains both MHC class II+ and MHC class II(-) cells. MHC class II+ Tregs belong to the integrin alpha(4)beta(1)+ subpopulation and exclusively execute contact-dependent suppressive activity. Here we present a method optimized for isolation of these MHC class II expressing Tregs from large leukaphereses products using magnetic microbeads that achieves a reproducible purity of more than 90% and enables the use of this small-sized Treg population in pre-clinical application and basic research.
2010 Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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CD4 Antigens / biosynthesis
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Cells, Cultured
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Flow Cytometry
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Forkhead Transcription Factors / biosynthesis
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High-Throughput Screening Assays
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Histocompatibility Antigens Class II / biosynthesis*
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Humans
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Immune Tolerance
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Immunomagnetic Separation*
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Integrin alpha4beta1 / biosynthesis*
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Interleukin-2 Receptor alpha Subunit / biosynthesis
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Leukapheresis
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T-Lymphocyte Subsets / immunology
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T-Lymphocyte Subsets / metabolism*
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T-Lymphocyte Subsets / pathology
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T-Lymphocytes, Regulatory / immunology
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T-Lymphocytes, Regulatory / metabolism*
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T-Lymphocytes, Regulatory / pathology
Substances
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CD4 Antigens
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FOXP3 protein, human
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Forkhead Transcription Factors
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Histocompatibility Antigens Class II
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Integrin alpha4beta1
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Interleukin-2 Receptor alpha Subunit