Abstract
A technique for the isolation of functional rat pancreatic mitochondria is described. The resultant mitochondrial preparations contained oligomycin-insensitive Mg2+-ATPase activity and coupled respiration could only be demonstrated in the absence of Mg2+ and in the presence of EDTA.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenosine Triphosphatases / metabolism
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Animals
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Ca(2+) Mg(2+)-ATPase
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Cell Fractionation / methods
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Edetic Acid / pharmacology
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In Vitro Techniques
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Magnesium / pharmacology
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Male
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Mitochondria / enzymology
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Mitochondria / metabolism
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Mitochondria / ultrastructure*
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Oligomycins / pharmacology
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Oxygen Consumption / drug effects
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Pancreas / metabolism
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Pancreas / ultrastructure*
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Rats
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Rats, Inbred Strains
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Succinate Dehydrogenase / metabolism
Substances
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Oligomycins
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Edetic Acid
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Succinate Dehydrogenase
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Adenosine Triphosphatases
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Ca(2+) Mg(2+)-ATPase
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Magnesium