Abstract
Notch belongs to a family of transmembrane proteins that are widely conserved from flies to vertebrates and are thought to be involved in cell-fate decisions. In Drosophila, the Suppressor of hairless (Su(H)) gene and genes of the Enhancer of split (E(Spl)) complex, which encode proteins of the basic helix-loop-helix type have been implicated in the Notch signalling pathway. Mammalian homologues of E(Spl), such as the mouse Hairy enhancer of split (HES-1), have been isolated. Both HES-1 and the intracellular domain of murine Notch (mNotch) are able to block MyoD-induced myogenesis. Here we show that activated forms of mNotch associate with the human analogue of Su(H), KBF2/RBP-J kappa (refs 8,9) and act as transcriptional activators through the KBF2-binding sites of the HES-1 promoter.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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Basic Helix-Loop-Helix Transcription Factors
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Cell Line
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DNA-Binding Proteins / metabolism
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Drosophila
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Drosophila Proteins*
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HeLa Cells
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Homeodomain Proteins / genetics
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Humans
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Immunoglobulin J Recombination Signal Sequence-Binding Protein
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Membrane Proteins / metabolism*
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Mice
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Molecular Sequence Data
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Mutagenesis
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Nuclear Proteins*
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Oligodeoxyribonucleotides
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Promoter Regions, Genetic
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Receptors, Notch
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Repressor Proteins / genetics
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Transcription Factor HES-1
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Transcription Factors / metabolism*
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Transcriptional Activation*
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Transfection
Substances
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Basic Helix-Loop-Helix Transcription Factors
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DNA-Binding Proteins
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Drosophila Proteins
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Hes1 protein, mouse
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Homeodomain Proteins
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Immunoglobulin J Recombination Signal Sequence-Binding Protein
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Membrane Proteins
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N protein, Drosophila
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Nuclear Proteins
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Oligodeoxyribonucleotides
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RBPJ protein, human
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Rbpj protein, mouse
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Receptors, Notch
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Repressor Proteins
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Su(H) protein, Drosophila
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Transcription Factor HES-1
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Transcription Factors
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HES1 protein, human