Abstract
A novel cationic surfactant solution is used to lyse blood cells and precipitate RNA and DNA. The RNA is recovered by extracting the pellet with a small volume of guanidinium isothiocyanate or formamide.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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DNA / blood
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DNA / isolation & purification
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Genes, abl
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Humans
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Indicators and Reagents
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Leukemia, Myelogenous, Chronic, BCR-ABL Positive
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Molecular Sequence Data
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Oligodeoxyribonucleotides
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Oncogenes
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Polymerase Chain Reaction / methods*
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RNA / blood*
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RNA / genetics
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RNA / isolation & purification*
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RNA-Directed DNA Polymerase
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Surface-Active Agents
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Tumor Cells, Cultured
Substances
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Indicators and Reagents
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Oligodeoxyribonucleotides
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Surface-Active Agents
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RNA
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DNA
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RNA-Directed DNA Polymerase