Abstract
Epidermal growth factor (EGF) modulates several functions of human enterocytes. We report that this growth factor induces strong tyrosine phosphorylation stimulation of its receptor and several putative substrates of the receptor intrinsic kinase including c-erb B2 in proliferating human colon carcinoma cells (Caco-2). In addition EGF induces stable association of the GTP-ase activating protein of p21ras to the p190 protein and to a 62 mol.wt. tyrosine-phosphorylated protein. This association is probably consequent to EGF stimulation of protein tyrosine phosphorylation and could coordinate progression through cell cycle with polarity, cell-cell interactions and cell mobility.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antibodies
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Blotting, Western
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Epidermal Growth Factor / immunology
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Epidermal Growth Factor / physiology*
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GTPase-Activating Proteins
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Guanine Nucleotide Exchange Factors*
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Humans
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Nuclear Proteins / metabolism*
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Phosphoproteins / metabolism*
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Phosphorylation
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Proteins / metabolism*
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Proto-Oncogene Proteins p21(ras) / metabolism
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Receptor, ErbB-2 / metabolism
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Repressor Proteins
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Tumor Cells, Cultured
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Tyrosine / immunology
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Tyrosine / metabolism*
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ras GTPase-Activating Proteins
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ras-GRF1
Substances
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ARHGAP35 protein, human
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Antibodies
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GTPase-Activating Proteins
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Guanine Nucleotide Exchange Factors
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Nuclear Proteins
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Phosphoproteins
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Proteins
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RASGRF1 protein, human
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Repressor Proteins
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ras GTPase-Activating Proteins
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ras-GRF1
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Tyrosine
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Epidermal Growth Factor
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Receptor, ErbB-2
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HRAS protein, human
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Proto-Oncogene Proteins p21(ras)