Abstract
Here we report the cDNA sequence of human Gal beta 1,3(4)GlcNAc alpha 2,3-sialyltransferase. The cDNA was isolated from a human placental cDNA library by screening with a 150bp probe generated by PCR using degenerate primers based on the sequences found in the sialyl motif. Comparative analysis of this cDNA with the rat liver alpha 2,3-sialyltransferase sequence indicates 91% nucleotide similarity between the two sequence in the predicted coding region. On the amino acid level, the degree of conservation is 97%. Surprisingly, Northern analysis indicated that the gene is expressed at low levels in human placenta but is abundantly expressed in skeletal muscle and fetal tissues.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Base Sequence
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Carbohydrate Sequence
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Cell Line
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Cloning, Molecular
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Female
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Gene Expression
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Gene Library
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Humans
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Kinetics
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Molecular Sequence Data
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Oligodeoxyribonucleotides
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Oligonucleotide Probes
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Placenta / enzymology
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Polymerase Chain Reaction
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Pregnancy
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Rats
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Sequence Homology, Amino Acid
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Sialyltransferases / biosynthesis*
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Sialyltransferases / genetics
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Sialyltransferases / metabolism
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Substrate Specificity
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Transfection
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beta-Galactoside alpha-2,3-Sialyltransferase
Substances
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Oligodeoxyribonucleotides
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Oligonucleotide Probes
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Sialyltransferases
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beta-Galactoside alpha-2,3-Sialyltransferase
Associated data
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GENBANK/L16870
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GENBANK/L16871
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GENBANK/L16872
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GENBANK/L16873
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GENBANK/L16874
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GENBANK/L16875
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GENBANK/L16876
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GENBANK/L16877
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GENBANK/L16878
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GENBANK/L23768