Abstract
The promoter of the human gene encoding the p65 subunit of the transcription factor NF-kappa B was cloned and the nucleotide sequence determined. The p65 promoter lacks both TATA and CCAAT consensus sequences. The p65 promoter contains three consensus binding sites of the transcription factor SP1. In contrast to the promoter of the p50 subunit of NF-kappa B, no sequences predicted to bind NF-kappa B are present in the p65 promoter. Phorbol ester (PMA) and phytohemagglutinin (PHA) treatment of Jurkat cells did not activate the p65 promoter in transient transfection experiments. Using different deletion mutants of the p65 promoter, essential promoter elements were mapped.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Base Sequence
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Binding Sites
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Chloramphenicol O-Acetyltransferase / biosynthesis
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Chloramphenicol O-Acetyltransferase / genetics
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Consensus Sequence
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Enzyme Activation / drug effects
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Humans
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Leukemia, T-Cell
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Molecular Sequence Data
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NF-kappa B / chemistry*
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NF-kappa B / drug effects
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NF-kappa B / genetics
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NF-kappa B / metabolism
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NF-kappa B p50 Subunit
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Phytohemagglutinins / pharmacology
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Protein Precursors / chemistry
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Protein Precursors / drug effects
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Tetradecanoylphorbol Acetate / pharmacology
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Transcription Factor RelA
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Tumor Cells, Cultured
Substances
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NF-kappa B
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NF-kappa B p50 Subunit
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Phytohemagglutinins
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Protein Precursors
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Transcription Factor RelA
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Chloramphenicol O-Acetyltransferase
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Tetradecanoylphorbol Acetate