Abstract
Stathmin is a cytosolic phosphoprotein previously described as a ubiquitous relay integrating various signaling pathways. It is the generic element of a protein family in mammals as in Xenopus, including SCG10, a neuron-specific, growth-associated protein, and RB3/XB3, related to the expression of differentiated functions of mature cells of the nervous system. In an extensive search for other members of the stathmin family, we identified cDNAs coding for two novel stathmin-related proteins: (a) a cDNA from a rat striatum cDNA library codes for RB3", a splice variant of RB3, with an additional basic domain in its N-terminal region; and (b) another cDNA identified through a systematic search in EST databases codes for a novel protein, SCLIP, for "SCG10-like protein," displaying 70% identity with SCG10 and sharing the same domain organization, with an N-terminal domain likely involved in membrane attachment and a C-terminal stathmin-like domain. Northern blot analysis as well as in situ hybridization on 14-day rat embryos showed that SCLIP mRNA is expressed only in neural structures. SCLIP mRNA is expressed at comparable levels in neonatal and adult rat brain, suggesting a potential role not only in the acquisition, but also in the expression of differentiated neuronal functions.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Alternative Splicing
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Amino Acid Sequence
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Animals
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Base Sequence
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Blotting, Northern
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Calcium-Binding Proteins
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Carrier Proteins
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DNA, Complementary / chemistry
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DNA, Complementary / genetics
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Gene Expression Regulation
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Gene Library
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Humans
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In Situ Hybridization
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Intracellular Signaling Peptides and Proteins
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Male
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Membrane Proteins
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Mice
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Mice, Inbred BALB C
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Microtubule Proteins*
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Middle Aged
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Molecular Sequence Data
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Nerve Growth Factors / biosynthesis
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Nerve Growth Factors / chemistry
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Nerve Growth Factors / genetics*
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Nervous System / embryology
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Nervous System / metabolism
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PC12 Cells
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Phosphoproteins / biosynthesis
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Phosphoproteins / chemistry
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Phosphoproteins / genetics*
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RNA, Messenger / biosynthesis
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Rats
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Species Specificity
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Stathmin
Substances
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Calcium-Binding Proteins
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Carrier Proteins
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DNA, Complementary
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Intracellular Signaling Peptides and Proteins
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Membrane Proteins
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Microtubule Proteins
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Nerve Growth Factors
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Phosphoproteins
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RNA, Messenger
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STMN1 protein, human
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STMN2 protein, human
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STMN3 protein, human
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STMN4 protein, human
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Stathmin
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Stmn1 protein, mouse
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Stmn2 protein, mouse
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Stmn2 protein, rat
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Stmn3 protein, mouse
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Stmn3 protein, rat
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Stmn4 protein, mouse
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Stmn4 protein, rat
Associated data
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GENBANK/AF026530
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GENBANK/AF069708
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GENBANK/AF069709
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GENBANK/AF069710