User profiles for "author:Nick Barker"

Prof Nick Barker

A-STAR Institute of Molecular and Cellular Biology, Singapore
Verified email at imcb.a-star.edu.sg
Cited by 58318

Adult intestinal stem cells: critical drivers of epithelial homeostasis and regeneration

N Barker - Nature reviews Molecular cell biology, 2014 - nature.com
Small populations of adult stem cells are responsible for the remarkable ability of the
epithelial lining of the intestine to be efficiently renewed and repaired throughout life. The …

Organoids as an in vitro model of human development and disease

A Fatehullah, SH Tan, N Barker - Nature cell biology, 2016 - nature.com
The in vitro organoid model is a major technological breakthrough that has already been
established as an essential tool in many basic biology and clinical applications. This near …

Mining the Wnt pathway for cancer therapeutics

N Barker, H Clevers - Nature reviews Drug discovery, 2006 - nature.com
Aberrant activation of the Wnt pathway is implicated in driving the formation of various
human cancers, particularly those of the digestive tract. Inhibition of aberrant Wnt pathway …

Identification of stem cells in small intestine and colon by marker gene Lgr5

N Barker, JH Van Es, J Kuipers, P Kujala… - Nature, 2007 - nature.com
The intestinal epithelium is the most rapidly self-renewing tissue in adult mammals. It is
currently believed that four to six crypt stem cells reside at the+ 4 position immediately above …

Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche

T Sato, RG Vries, HJ Snippert, M Van De Wetering… - Nature, 2009 - nature.com
The intestinal epithelium is the most rapidly self-renewing tissue in adult mammals. We have
recently demonstrated the presence of about six cycling Lgr5+ stem cells at the bottoms of …

Activation of β-catenin-Tcf signaling in colon cancer by mutations in β-catenin or APC

PJ Morin, AB Sparks, V Korinek, N Barker, H Clevers… - Science, 1997 - science.org
Inactivation of the adenomatous polyposis coli (APC) tumor suppressor gene initiates
colorectal neoplasia. One of the biochemical activities associated with the APC protein is …

Constitutive Transcriptional Activation by a β-Catenin-Tcf Complex in APC−/− Colon Carcinoma

V Korinek, N Barker, PJ Morin, D Van Wichen… - Science, 1997 - science.org
The adenomatous polyposis coli (APC) tumor suppressor protein binds to β-catenin, a
protein recently shown to interact with Tcf and Lef transcription factors. The gene encoding …

[PDF][PDF] Lgr5+ ve stem cells drive self-renewal in the stomach and build long-lived gastric units in vitro

N Barker, M Huch, P Kujala, M van de Wetering… - Cell stem cell, 2010 - cell.com
The study of gastric epithelial homeostasis and cancer has been hampered by the lack of
stem cell markers and in vitro culture methods. The Wnt target gene Lgr5 marks stem cells in …

Paneth cells constitute the niche for Lgr5 stem cells in intestinal crypts

T Sato, JH Van Es, HJ Snippert, DE Stange, RG Vries… - Nature, 2011 - nature.com
Homeostasis of self-renewing small intestinal crypts results from neutral competition
between Lgr5 stem cells, which are small cycling cells located at crypt bottoms,. Lgr5 stem …

Crypt stem cells as the cells-of-origin of intestinal cancer

N Barker, RA Ridgway, JH Van Es, M Van De Wetering… - Nature, 2009 - nature.com
Intestinal cancer is initiated by Wnt-pathway-activating mutations in genes such as
adenomatous polyposis coli (APC). As in most cancers, the cell of origin has remained …