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Ferrous iron uptake byBifidobacterium breve

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Abstract

Bifidobacterium breve transports ferrous iron in preference to the ferric form in a saturable, concentration-dependent manner with an optimum pH of 6. Iron transport is highly temperature sensitive. Two transport systems with apparent Km’s of 86±27 and 35±20 μM (p>0.01) were distinguished, one operating at high iron concentrations, the other at low iron concentrations. Iron uptake could not be accounted for by surface binding. Uptake of iron was inhibited by iron chelators, a proton ionophore, and ATPase inhibitors, and it was stimulated by potassium ionophores. The presence of a ferri reductase in the insoluble cell fraction ofB. breve and its “spent” growth medium was demonstrated. The hypothesis is presented that iron uptake by bifidobacteria is related to the nutritional immunity phenomenon.

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References

  1. J. B. Neilands,Ann. Rev. Nutr. 1, 27 (1981).

    Article  CAS  Google Scholar 

  2. A. Bezkorovainy, N. Topouzian, and R. Miller-Catchpole,Clin. Physiol. Biochem. 4, 150 (1986).

    PubMed  CAS  Google Scholar 

  3. J. S. Lodge and T. Emery,J. Bact. 160, 801 (1984).

    PubMed  CAS  Google Scholar 

  4. S. L. Evans, J. E. L. Arceneaux, B. R. Byers, M. E. Martin, and H. Aranha,J. Bact. 168, 1096 (1986).

    PubMed  CAS  Google Scholar 

  5. A. Bezkorovainy, R. Miller-Catchpole, M. Poch, and L. Solberg,Biochim. Biophys. Acta 884, 60 (1986).

    CAS  Google Scholar 

  6. A. Bezkorovainy, L. Solberg, M. Poch, and R. Miller-Catchpole,Int. J. Biochem. 19, 517 (1987).

    Article  PubMed  CAS  Google Scholar 

  7. B. Biavati, P. Castagnoli, F. Crociani, and L. D. Trovatelli,Microbiologica 7, 341 (1984).

    PubMed  CAS  Google Scholar 

  8. G. Reuter,Zbl. Bakt. Abt. I., Orig. 191, 486 (1963).

    CAS  Google Scholar 

  9. Y. Benno, K. Sawada, and T. Mitsuoka,Microbiol. Immunol. 28, 975 (1984).

    PubMed  CAS  Google Scholar 

  10. H. Beerens, C. Romond, and C. Neut,Amer. J. Clin. Nutr. 33, 2434 (1980).

    PubMed  CAS  Google Scholar 

  11. T. Mitsuoka and C. Kaneuchi,Amer. J. Clin. Nutr. 30, 1799 (1977).

    PubMed  CAS  Google Scholar 

  12. C. L. Bullen, P. V. Tearle, and A. T. Willis,J. Med. Microbiol. 9, 325 (1976).

    Article  PubMed  CAS  Google Scholar 

  13. H. Haenel, W. Müller-Beuthow, and F. K. Grütte,Zbl. Bakt., I. Abt. Orig. 215, 333 (1970).

    CAS  Google Scholar 

  14. C. L. Bullen and P. V. Tearle,J. Med. Microbiol. 9, 335 (1976).

    PubMed  CAS  Google Scholar 

  15. A. Bezkorovainy, L. Solberg, R. Miller-Catchpole, and M. Poch,Biol. Trace Elem. Res. 17, 123–137 (1988).

    Article  PubMed  CAS  Google Scholar 

  16. A. P. Pugsley and P. Reeves,J. Bact. 130, 26 (1977).

    PubMed  CAS  Google Scholar 

  17. R. Serrano,Eur. J. Biochem. 105, 419 (1980).

    Article  PubMed  CAS  Google Scholar 

  18. R. M. Dharmavaram, and J. Konisky,J. Bact. 169, 3921 (1987).

    PubMed  CAS  Google Scholar 

  19. J. H. Veerkamp,Antonie van Leeuwenhoek 43, 111 (1977).

    Article  PubMed  CAS  Google Scholar 

  20. J. H. Veerkamp,Microbios 22, 35 (1978).

    PubMed  CAS  Google Scholar 

  21. I. D. Ryabova, G. A. Gorneva, and Yu. O. Ovchinnikov,Biochim. Biophys. Acta 401, 109 (1975).

    Article  PubMed  CAS  Google Scholar 

  22. N. Topouzian and A. Bezkorovainy,IRCS Med. Sci. 14, 275 (1986).

    CAS  Google Scholar 

  23. A. Bezkorovainy and N. Topouzian,Int. J. Biochem. 15, 361 (1983).

    Article  CAS  Google Scholar 

  24. E. D. Weinberg,Clin. Physiol. Biochem. 4, 50 (1986).

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Biochemistry, Rush Presbyterian-St. Luke’s Medical Center, 60612, Chicago, IL

    Anatoly Bezkorovainy & Leslie Solberg

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  1. Anatoly Bezkorovainy
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  2. Leslie Solberg
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Bezkorovainy, A., Solberg, L. Ferrous iron uptake byBifidobacterium breve . Biol Trace Elem Res 20, 251–267 (1989). https://doi.org/10.1007/BF02917440

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  • DOI: https://doi.org/10.1007/BF02917440

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