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PWE-169 Analysis of faecal volatiles from young children infected with and without rotavirus
  1. H Al-Kateb1,
  2. N Cunliffe2,
  3. B deLacy Costello1,
  4. C Probert2,
  5. N Ratcliffe1
  1. 1Applied sciences, University of West of England/Centre of Research in Biosciences, Coldharbour Lane, Bristol, UK
  2. 2Department of Gastroenterology, Institute of Tanslational Medicine, Liverpool, UK


Introduction Rotavirus is the most common cause of severe diarrhoea among infants and young children. Rotavirus is usually an easily managed disease of childhood, but worldwide nearly 500 000 children under 5 years of age still die from rotavirus infection each year and almost two million more become severely ill. Rotavirus A (responsible for about 90% of infections) is typically diagnosed by finding the virus in the child's stool by enzyme immunoassay. This study was undertaken to expand our knowledge of VOCs from the stool of children and assess whether rotavirus causes easily measurable changes in the gut chemistry of infected young children.

Methods The volatile organic compounds (VOCs) from the stool of 53 children from Malawi (26 non-infected children with an unspecified GI problem and 27 rotavirus children diagnosed with the virus) were analysed using Headspace Trap-GC/MS. The faecal samples were placed in headspace vials and were heated from frozen to 90°C. The VOC's were preconcentrated and focused prior to GC/MS analysis. Those VOCs were identified by comparing their mass spectra with those contained in the NIST/EPA/NIH Mass Spectral Database.

Results A total of 186 different compounds have so far been identified. Of the 53 stool samples ethanol was found in 69% and 63% samples respectively between the non and infected classes, which contrasts with previous work where ethanol was found in all healthy adult and all healthy neonate stool. This could be due to the more dilute stool due to diarrhoea. Carbon disulphide has previously been found to be ubiquitous in healthy adult stool, again the frequency was much less at 27% and 22% respectively. In contrast the majority of samples contained ethanoic acid, with more samples in the rotavirus group. There were very little differences in the frequency (ca. 92%) and abundance of ethanol in both sample classes, curiously ethanol has been previously shown to be present in all adult samples and absent in neonates. In contrast 2,3-butanedione and other aldehydes were significantly present both at greater frequency and typically at higher concentrations in rotavirus samples compared to non-rota samples, see Abstract PWE-169 figure 1.

Abstract PWE-169 Figure 1

Combined peak area (arbitrary units) of selected carbonyl compounds (namely; 2,3-butanedione/propanal/2-heptenal, (Z)-/octanal/nonanal combined) present in faecal samples analysed.

Conclusion Very little work has been published on volatile compounds from stool in particular from the stool of children, this work adds to this knowledge. Some compounds such as ethanol were found in approx. equal amounts in the diarrhoea of infected and non-infected stool, however in general there was a greater frequency and abundance of VOCs in the rota infected samples, particularly of aldehydes and 2,3-butandione.

Competing interests None declared.

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